Any feedback?
Literature summary for 2.3.1.81 extracted from
- Kinetic mechanisms of gentamicin acetyltransferase I. Antibiotic-dependent shift from rapid to nonrapid equilibrium random mechanisms (1978), J. Biol. Chem., 253, 5902-5907.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyltobramycin | - |
Escherichia coli |  |
| amikacin | - |
Escherichia coli | |
| butyryl-CoA | dead-end inhibition pattern | Escherichia coli | |
| CoA | product inhibition | Escherichia coli | |
| gentamicin C1a | substrate inhibition | Escherichia coli | |
| additional information | inhibition kinetics | Escherichia coli | |
| N3-Acetyltobramycin | product inhibition | Escherichia coli | |
| neomycin | - |
Escherichia coli | |
| ribostamycin | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic pattern | Escherichia coli | |
| 0.00012 | - |
gentamicin C1a | - |
Escherichia coli | |
| 0.0013 | - |
acetyl-CoA | cosubstrate gentamicin C1a | Escherichia coli | |
| 0.0016 | - |
tobramycin | - |
Escherichia coli | |
| 0.0017 | - |
acetyl-CoA | cosubstrate tobramycin | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
containing R-factor JR88 | - |
| Escherichia coli C600 | - |
containing R-factor JR88 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + gentamicin C1a | - |
Escherichia coli | CoA + N3'-acetylgentamicin C1a | - |
? | |
| acetyl-CoA + gentamicin C1a | - |
Escherichia coli C600 | CoA + N3'-acetylgentamicin C1a | - |
? | |
| acetyl-CoA + sisomicin | - |
Escherichia coli | CoA + N3'-acetylsisomicin | - |
? | |
| acetyl-CoA + sisomicin | - |
Escherichia coli C600 | CoA + N3'-acetylsisomicin | - |
? | |
| acetyl-CoA + tobramycin | - |
Escherichia coli | CoA + N3'-acetyltobramycin | - |
? | |
| acetyl-CoA + tobramycin | - |
Escherichia coli C600 | CoA + N3'-acetyltobramycin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
