Protein Variants | Comment | Organism |
---|---|---|
D99A | 39fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 215fold decrease in the ratio of turnover number to Km-value for kanamycin A, 86fold decrease in the ratio of turnover number to Km-value for neamine | Enterococcus sp. |
D99E | 4.7fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 31fold decrease in the ratio of turnover number to Km-value for kanamycin A, 19.3fold decrease in the ratio of turnover number to Km-value for neamine, 134fold decrease in the ratio of turnover number to Km-value for fortimicin A | Enterococcus sp. |
D99N | 7fold decrease in the ratio of turnover number to Km-value for acetyl-CoA, 130fold decrease in the ratio of turnover number to Km-value for kanamycin A, 102fold decrease in the ratio of turnover number to Km-value for neamine | Enterococcus sp. |
Y96F | minor effects on the steady state kinetic parameters, largest effects for paromomycin, 5.5fold decrease in the ratio kcat /Km compared with wild-type | Enterococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(bromomethyl)phenanthrene | inactivates through covalent modification of Asp99 | Enterococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.0254 | - |
fortimicin A | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.031 | - |
kanamycin A | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.038 | - |
acetyl-CoA | pH 7.5, 37°C,wild-type AAC(6')-Ie | Enterococcus sp. | |
0.0399 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.0435 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
0.0453 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.07 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.0736 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
0.078 | - |
neamine | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.079 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
0.0902 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.102 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.229 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.324 | - |
paromomycin | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.326 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
0.443 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.509 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.771 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.771 | - |
paromomycin | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + fortimicin A | - |
Enterococcus sp. | CoA + N6'-acetylfortimicin A | - |
? | |
acetyl-CoA + kanamycin A | - |
Enterococcus sp. | CoA + N6'-acetylkanamycin A | - |
? | |
acetyl-CoA + neamine | - |
Enterococcus sp. | CoA + N6'-acetylneamine | - |
? | |
acetyl-CoA + paromomycin | - |
Enterococcus sp. | CoA + O6'-acetylparomomycin | - |
? | |
additional information | Asp-88 acts as an active site base in the molecular mechanism of AAC(6')-Ie | Enterococcus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAC(6')-Ie | aminoglycoside acetyltransferase domain of acetyltransferase-6'-aminoglycoside phosphotransferase-2" | Enterococcus sp. |
acetyltransferase-6'-aminoglycoside phosphotransferase-2" | - |
Enterococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.0004 | - |
paromomycin | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.0008 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.006 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.026 | - |
paromomycin | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
0.028 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.029 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.033 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.035 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99A | Enterococcus sp. | |
0.038 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.059 | - |
paromomycin | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.066 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.11 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.17 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie D99N | Enterococcus sp. | |
0.19 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie D99E | Enterococcus sp. | |
0.28 | - |
fortimicin A | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.43 | - |
neamine | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
0.45 | - |
fortimicin A | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
1.1 | - |
kanamycin A | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
1.2 | - |
acetyl-CoA | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. | |
1.3 | - |
acetyl-CoA | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
1.3 | - |
neamine | pH 7.5, 37°C, mutant AAC(6')-Ie Y96F | Enterococcus sp. | |
1.7 | - |
kanamycin A | pH 7.5, 37°C, wild-type AAC(6')-Ie | Enterococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
wild-type AAC(6')-Ie with acetyl-CoA or neamine as variable substrate | Enterococcus sp. |