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Literature summary for 2.3.1.82 extracted from

  • Green, K.; Garneau-Tsodikova, S.
    Domain dissection and characterization of the aminoglycoside resistance enzyme ANT(3'')-Ii/AAC(6')-IId from Serratia marcescens (2013), Biochimie, 95, 1319-1325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Serratia marcescens

Protein Variants

Protein Variants Comment Organism
additional information separation of the 3''-adenylyltransferase-aminoglycoside and 6'-acetyltransferase domains of the bifunctional enzyme, and comparison of their activity to that of the full-length enzyme Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
amikacin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.00177
-
netilmicin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.00202
-
kanamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.0022
-
sisomicin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.00387
-
netilmicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.0049
-
sisomicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.00504
-
gentamicin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.0079
-
tobramycin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.0143
-
gentamicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.0148
-
tobramycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.016
-
ribostamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.016
-
kanamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.023
-
neomycin full length enzyme, pH 7.5, 25°C Serratia marcescens
0.0321
-
neomycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.0693
-
ribostamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.127
-
amikacin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.147
-
acetyl-CoA isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
0.215
-
acetyl-CoA full length enzyme, pH 7.5, 25°C Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens Q8VQN7 bifunctional 3''-adenylyltransferase-aminoglycoside and 6'-acetyltransferase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
Serratia marcescens CoA + 6'-N-acetylamikacin
-
?
acetyl-CoA + gentamicin
-
Serratia marcescens CoA + 6'-N-acetylgentamicin
-
?
acetyl-CoA + kanamycin
-
Serratia marcescens CoA + 6'-N-acetylkanamycin
-
?
acetyl-CoA + neomycin
-
Serratia marcescens CoA + 6'-N-acetylneomycin
-
?
acetyl-CoA + netilmicin
-
Serratia marcescens CoA + 6'-N-acetylnetilmicin
-
?
acetyl-CoA + ribostamycin
-
Serratia marcescens CoA + 6'-N-acetylribostamycin
-
?
acetyl-CoA + sisomicin
-
Serratia marcescens CoA + 6'-N-acetylsisomicin
-
?
acetyl-CoA + tobramycin
-
Serratia marcescens CoA + 6'-N-acetyltobramycin
-
?

Synonyms

Synonyms Comment Organism
ANT(3'')-Ii/AAC(6')-IId
-
Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11
-
gentamicin full length enzyme, pH 7.5, 25°C Serratia marcescens
1.62
-
acetyl-CoA full length enzyme, pH 7.5, 25°C Serratia marcescens
1.91
-
netilmicin full length enzyme, pH 7.5, 25°C Serratia marcescens
2.13
-
kanamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
2.92
-
amikacin full length enzyme, pH 7.5, 25°C Serratia marcescens
2.93
-
sisomicin full length enzyme, pH 7.5, 25°C Serratia marcescens
3.21
-
netilmicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
3.33
-
gentamicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
3.73
-
tobramycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
3.81
-
kanamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
3.84
-
tobramycin full length enzyme, pH 7.5, 25°C Serratia marcescens
3.88
-
ribostamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
5.35
-
sisomicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
5.7
-
ribostamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
7.09
-
amikacin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
9.06
-
neomycin full length enzyme, pH 7.5, 25°C Serratia marcescens
11.1
-
neomycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
12.1
-
acetyl-CoA isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.4
-
acetyl-CoA full length enzyme, pH 7.5, 25°C Serratia marcescens
62.4
-
amikacin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
82
-
acetyl-CoA isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
82.2
-
ribostamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
133
-
kanamycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
189
-
kanamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
220
-
gentamicin full length enzyme, pH 7.5, 25°C Serratia marcescens
234
-
gentamicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
243
-
ribostamycin full length enzyme, pH 7.5, 25°C Serratia marcescens
252
-
tobramycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
346
-
neomycin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
394
-
neomycin full length enzyme, pH 7.5, 25°C Serratia marcescens
485
-
tobramycin full length enzyme, pH 7.5, 25°C Serratia marcescens
829
-
netilmicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
1080
-
netilmicin full length enzyme, pH 7.5, 25°C Serratia marcescens
1090
-
sisomicin isolated AAC(6') domain, pH 7.5, 25°C Serratia marcescens
1330
-
sisomicin full length enzyme, pH 7.5, 25°C Serratia marcescens
5250
-
amikacin full length enzyme, pH 7.5, 25°C Serratia marcescens