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Literature summary for 2.3.1.87 extracted from

  • Ferry, G.; Ubeaud, C.; Dauly, C.; Mozo, J.; Guillard, S.; Berger, S.; Jimenez, S.; Scoul, C.; Leclerc, G.; Yous, S.; Delagrange, P.; Boutin, J.A.
    Purification of the recombinant human serotonin N-acetyltransferase (EC 2.3.1.87): further characterization of and comparison with AANAT from other species (2004), Protein Expr. Purif., 38, 84-98.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
baculovirus infection of Tn5 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA-tryptamine IC50: 0.00041 mM, GST-AANAT fusion protein Homo sapiens
acetyl-CoA-tryptamine IC50: 0.00062 mM, GST-AANAT fusion protein Ovis aries
acetyl-CoA-tryptamine IC50: 0.0008 mM, GST-AANAT fusion protein Rattus norvegicus
bromoacetyltryptamine IC50: 0.00143 mM, GST-AANAT fusion protein Homo sapiens
bromoacetyltryptamine IC50: 0.00128 mM, GST-AANAT fusion protein Ovis aries
bromoacetyltryptamine IC50: 0.0051 mM, GST-AANAT fusion protein Rattus norvegicus
S 20251 IC50: 0.002 mM, GST-AANAT fusion protein Homo sapiens
S 20251 IC50: 0.0013 mM, GST-AANAT fusion protein Ovis aries
S 20251 IC50: 0.0024 mM, GST-AANAT fusion protein Rattus norvegicus
S 23823 IC50: 0.005 mM, GST-AANAT fusion protein Homo sapiens
S 23823 IC50: 0.031 mM, GST-AANAT fusion protein Rattus norvegicus
S 27244 IC50: 0.00072 mM, GST-AANAT fusion protein Homo sapiens
S 27244 IC50: 0.00026 mM, GST-AANAT fusion protein Ovis aries
S 27244 IC50: 0.00036 mM, GST-AANAT fusion protein Rattus norvegicus
S 27481 IC50: 0.00018 mM, GST-AANAT fusion protein Homo sapiens
S 27481 IC50: 0.0004 mM, GST-AANAT fusion protein Ovis aries
S 27481 IC50: 0.0004 mM, GST-AANAT fusion protein Rattus norvegicus
S 28036 IC50: 0.0056 mM,GST-AANAT fusion protein Homo sapiens
S 28036 IC50: 0.00073 mM, GST-AANAT fusion protein Ovis aries
S 28036 IC50: 0.0017 mM, GST-AANAT fusion protein Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
2-Phenylethylamine pH 6.8, 37°C Homo sapiens
0.265
-
acetyl-CoA pH 6.8, 37°C Ovis aries
0.34
-
2-(2,3-dichlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
0.47
-
2,5-dimethoxyphenylethylamine pH 6.8, 37°C Ovis aries
0.49
-
2-methoxyphenylethylamine pH 6.8, 37°C Ovis aries
0.53
-
acetyl-CoA pH 6.8, 37°C Homo sapiens
0.62
-
2-(3-chlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
0.64
-
serotonin pH 6.8, 37°C Ovis aries
0.65
-
acetyl-CoA pH 6.8, 37°C Rattus norvegicus
0.75
-
2-(4-bromophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
0.9
-
2-(2-chlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
0.98
-
2-(p-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
1.1
-
2-(3-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
1.17
-
2-(p-tolyl)-ethylamine pH 6.8, 37°C Homo sapiens
1.28
-
2-(4-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.3
-
2-methoxyphenylethylamine pH 6.8, 37°C Homo sapiens
1.31
-
2-(3-chlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.35
-
serotonin pH 6.8, 37°C Homo sapiens
1.37
-
2-fluorophenylethylamine pH 6.8, 37°C Ovis aries
1.37
-
2-fluorophenylethylamine pH 6.8, 37°C Homo sapiens
1.405
-
2-(p-nitrophenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.55
-
2-(4-bromophenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.58
-
2-(p-tolyl)-ethylamine pH 6.8, 37°C Ovis aries
1.65
-
2-(3-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.7
-
serotonin pH 6.8, 37°C Rattus norvegicus
1.76
-
2-(3,4-dihydroxyphenyl)-ethylamine pH 6.8, 37°C Ovis aries
1.8
-
2-Phenylethylamine pH 6.8, 37°C Ovis aries
1.815
-
3-methoxytyramine pH 6.8, 37°C Ovis aries
1.9
-
6-Hydroxydopamine pH 6.8, 37°C Homo sapiens
1.91
-
2-(p-fluorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
2.07
-
3-methoxy-2-phenylethylamine pH 6.8, 37°C Ovis aries
2.2
-
2-(4-fluorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
2.33
-
2-(4-chlorophenyl)ethylamine pH 6.8, 37°C Ovis aries
2.74
-
2-(2-chlorophenyl)-ethylamine pH 6.8, 37°C Homo sapiens
3.46
-
2-(2,3-dichlorophenyl)-ethylamine pH 6.8, 37°C Ovis aries
9.5
-
2-Phenylethylamine pH 6.8, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16613
-
-
Ovis aries
-
-
-
Rattus norvegicus Q64666
-
-

Purification (Commentary)

Purification (Comment) Organism
denaturation/renaturation process in which the enzyme is immobilized on an affinity chromatography column Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.17
-
-
Ovis aries
10.57
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2,5-dimethoxyphenylethylamine 24% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 2,5-dimethoxyphenylethylamine 68% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2,5-dimethoxyphenylethylamine
-
?
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine 78% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(2,3-dichlorophenyl)-ethylamine 84% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(2,3-dichlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine 102% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(2-chlorophenyl)-ethylamine 130% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(2-chlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine 64% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
-
?
acetyl-CoA + 2-(3,4-dihydroxyphenyl)-ethylamine 7% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3,4-dihydroxyphenyl)-ethylamine
-
?
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine 15% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
-
?
acetyl-CoA + 2-(3,4-dimethoxyphenyl)-ethylamine 4.4% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3,4-dimethoxyphenyl)-ethylamine
-
?
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine 110% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(3-chlorophenyl)-ethylamine 67% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3-chlorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine 65% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(3-fluorophenyl)-ethylamine 93% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(3-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(4-bromophenyl)-ethylamine 48% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
-
?
acetyl-CoA + 2-(4-bromophenyl)-ethylamine 87% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-bromophenyl)-ethylamine
-
?
acetyl-CoA + 2-(4-chlorophenyl)ethylamine 68% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
-
?
acetyl-CoA + 2-(4-chlorophenyl)ethylamine 91% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-chlorophenyl)ethylamine
-
?
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine 116% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(4-fluorophenyl)-ethylamine 60% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(4-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine 56% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(p-fluorophenyl)-ethylamine as active as with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-fluorophenyl)-ethylamine
-
?
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine 53% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
-
?
acetyl-CoA + 2-(p-nitrophenyl)-ethylamine 9% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-nitrophenyl)-ethylamine
-
?
acetyl-CoA + 2-(p-tolyl)-ethylamine 60% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-(p-tolyl)-ethylamine
-
?
acetyl-CoA + 2-(p-tolyl)-ethylamine 87% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-(p-tolyl)-ethylamine
-
?
acetyl-CoA + 2-fluorophenylethylamine 69% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 2-fluorophenylethylamine 52% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-fluorophenylethylamine
-
?
acetyl-CoA + 2-methoxyphenylethylamine 52% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-2-methoxyphenylethylamine
-
?
acetyl-CoA + 2-methoxyphenylethylamine 97% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-2-methoxyphenylethylamine
-
?
acetyl-CoA + 2-phenylethylamine
-
Ovis aries CoA + N-acetyl-2-phenylethylamine
-
?
acetyl-CoA + 2-phenylethylamine
-
Rattus norvegicus CoA + N-acetyl-2-phenylethylamine
-
?
acetyl-CoA + 2-phenylethylamine
-
Homo sapiens CoA + N-acetyl-2-phenylethylamine
-
?
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine 1% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 3-hydroxy-4-methoxyphenethylamine 7.2% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-hydroxy-4-methoxyphenethylamine
-
?
acetyl-CoA + 3-hydroxyphenethylamine 24% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 3-hydroxyphenethylamine 36% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-hydroxyphenethylamine
-
?
acetyl-CoA + 3-methoxy-2-phenylethylamine 23% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 3-methoxy-2-phenylethylamine 55% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-methoxy-2-phenylethylamine
-
?
acetyl-CoA + 3-methoxytyramine 17% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 3-methoxytyramine 73% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-3-methoxytyramine
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonamide 1% of the activity with 2-phenylethylamine Ovis aries CoA + ?
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride 7.4% of the activity with 2-phenylethylamine Ovis aries CoA + ?
-
?
acetyl-CoA + 4-(2-aminoethyl)-benzenesulfonyl fluoride 8% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 4-methoxyphenylethylamine 10% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-4-methoxyphenylethylamine
-
?
acetyl-CoA + 4-methoxyphenylethylamine 11% of the activity with 2-phenylethylamine Homo sapiens CoA + N-acetyl-4-methoxyphenylethylamine
-
?
acetyl-CoA + 5-hydroxydopamine 4.4% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-5-hydroxydopamine
-
?
acetyl-CoA + 6-hydroxydopamine 51% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
acetyl-CoA + 6-hydroxydopamine 1.5% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyl-6-hydroxydopamine
-
?
acetyl-CoA + serotonin
-
Ovis aries CoA + N-acetylserotonin
-
?
acetyl-CoA + serotonin
-
Rattus norvegicus CoA + N-acetylserotonin
-
?
acetyl-CoA + serotonin
-
Homo sapiens CoA + N-acetylserotonin
-
?
acetyl-CoA + tyramine 10% of the activity with 2-phenylethylamine Ovis aries CoA + N-acetyltyramine
-
?
acetyl-CoA + tyramine 8% of the activity with 2-phenylethylamine Homo sapiens CoA + ?
-
?
additional information no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine or 2-(p-chlorophenoxy)-2-methylpropionic acid Ovis aries ?
-
?
additional information no activity with 2-(4-aminophenyl)-ethylamine, 3,4-(dibenzyloxy)phenethylamine, 5-hydroxydopamine, 4-(2-aminoethyl)-benzenesulfonamide or 2-(p-chlorophenoxy)-2-methylpropionic acid Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
hAANAT
-
Homo sapiens
oAANAT
-
Ovis aries
oAANAT
-
Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00018
-
IC50: 0.00018 mM, GST-AANAT fusion protein Homo sapiens S 27481
0.00026
-
IC50: 0.00026 mM, GST-AANAT fusion protein Ovis aries S 27244
0.00036
-
IC50: 0.00036 mM, GST-AANAT fusion protein Rattus norvegicus S 27244
0.0004
-
IC50: 0.0004 mM, GST-AANAT fusion protein Ovis aries S 27481
0.0004
-
IC50: 0.0004 mM, GST-AANAT fusion protein Rattus norvegicus S 27481
0.00041
-
IC50: 0.00041 mM, GST-AANAT fusion protein Homo sapiens acetyl-CoA-tryptamine
0.00062
-
IC50: 0.00062 mM, GST-AANAT fusion protein Ovis aries acetyl-CoA-tryptamine
0.00072
-
IC50: 0.00072 mM, GST-AANAT fusion protein Homo sapiens S 27244
0.00073
-
IC50: 0.00073 mM, GST-AANAT fusion protein Ovis aries S 28036
0.0008
-
IC50: 0.0008 mM, GST-AANAT fusion protein Rattus norvegicus acetyl-CoA-tryptamine
0.00128
-
IC50: 0.00128 mM, GST-AANAT fusion protein Ovis aries bromoacetyltryptamine
0.0013
-
IC50: 0.0013 mM, GST-AANAT fusion protein Ovis aries S 20251
0.00143
-
IC50: 0.00143 mM, GST-AANAT fusion protein Homo sapiens bromoacetyltryptamine
0.0017
-
IC50: 0.0017 mM, GST-AANAT fusion protein Rattus norvegicus S 28036
0.002
-
IC50: 0.002 mM, GST-AANAT fusion protein Homo sapiens S 20251
0.0024
-
IC50: 0.0024 mM, GST-AANAT fusion protein Rattus norvegicus S 20251
0.005
-
IC50: 0.005 mM, GST-AANAT fusion protein Homo sapiens S 23823
0.0051
-
IC50: 0.0051 mM, GST-AANAT fusion protein Rattus norvegicus bromoacetyltryptamine
0.0056
-
IC50: 0.0056 mM,GST-AANAT fusion protein Homo sapiens S 28036
0.031
-
IC50: 0.031 mM, GST-AANAT fusion protein Rattus norvegicus S 23823