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Literature summary for 2.3.1.91 extracted from

  • Strack, D.; Knogge, W.; Dahlbender, B.
    Enzymatic synthesis of sinapine from 1-O-sinapoyl-beta-D-glucose and choline by a cell-free system from developing seeds of red radish (Raphanus sativus L. var. sativus) (1982), Z. Naturforsch. C, 38, 21-27.
No PubMed abstract available
Search for more literature for 2.3.1.91

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 1-O-sinapoyl-beta-D-glucose
-
 Raphanus sativus
7.64
-
 choline
-
 Raphanus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-sinapoyl-beta-D-glucose + choline Raphanus sativus
-
 D-glucose + sinapoylcholine
-
 ?

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
 var. sativus cv. Saxa
-

Source Tissue

Source Tissue Comment Organism Textmining
seed immature dark-green Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Tris buffer is unusable for enzyme assay due to formation of side products Raphanus sativus

Storage Stability

Storage Stability Organism
-20°C, crude extract, 1 week, loss of 20% activity Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-feruloyl-beta-D-glucose + choline 40% as active as 1-sinapoylglucose Raphanus sativus D-glucose + feruloylcholine
-
 ?
1-O-sinapoyl-beta-D-glucose + choline
-
 Raphanus sativus D-glucose + sinapoylcholine
-
 ?
1-O-sinapoyl-beta-D-glucose + choline absolute acceptor specificity towards choline Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
1-O-sinapoyl-beta-D-glucose + choline ethanolamine, myo-inositol, citric, L-malic, L-tartaric, quinic or shikimic acid are no acceptors Raphanus sativus D-glucose + sinapoylcholine i.e. sinapine ?
1-p-coumaroyl-beta-D-glucose + choline 13% Raphanus sativus D-glucose + p-coumaroylcholine
-
 ?

Synonyms

Synonyms Comment Organism
SCT
-
 Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 about Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
5.3 9 about half-maximal activity at pH 5.3 and pH 9.0 Raphanus sativus