Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Lupinus albus | |
dithiothreitol | 140% activity at 1 mM | Lupinus albus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-epilupinine | - |
Lupinus albus | |
(+)-lupanine | - |
Lupinus albus | |
CoA | competitive | Lupinus albus | |
N-ethylmaleimide | - |
Lupinus albus | |
p-chloromercuriphenyl sulfonic acid | - |
Lupinus albus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
13-alpha-hydroxymultiflorine | - |
Lupinus albus | |
0.027 | - |
13-hydroxylupanine | - |
Lupinus albus | |
0.46 | - |
tigloyl-CoA | with 13-alpha-hydroxymultiflorine | Lupinus albus | |
0.52 | - |
tigloyl-CoA | with 13-alpha-hydroxylupanine | Lupinus albus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
isoform A and B, gel filtration | Lupinus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tigloyl-CoA + 13-alpha-hydroxymultiflorine | Lupinus albus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxymultiflorine | - |
? | |
tigloyl-CoA + 13-alpha-hydroxymultiflorine | Lupinus albus | isoform A and B | CoA + 13-tigloyloxymultiflorine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | isoform A and B | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | Lupinus albus | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | CoA + 13-tigloyloxylupanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cytisus scoparius | - |
- |
- |
Lupinus albus | - |
bitter and sweet variant | - |
Lupinus albus | - |
2 isoforms A and B | - |
Lupinus hirsutus | - |
- |
- |
Lupinus polyphyllus x Lupinus arboreus | - |
- |
- |
no activity in Lupinus luteus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lupinus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Lupinus albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
isoform A | Lupinus albus |
2.18 | - |
isoform B | Lupinus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Lupinus albus | ? | - |
? | |
additional information | substrate specificity, overview | Lupinus albus | ? | - |
? | |
additional information | baptifoline, epilupinine and lupinine are no substrates | Lupinus albus | ? | - |
? | |
tigloyl-CoA + 13-alpha-hydroxymultiflorine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxymultiflorine | - |
? | |
tigloyl-CoA + 13-alpha-hydroxymultiflorine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus hirsutus | CoA + 13-tigloyloxymultiflorine | - |
? | |
tigloyl-CoA + 13-alpha-hydroxymultiflorine | isoform A and B | Lupinus albus | CoA + 13-tigloyloxymultiflorine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Cytisus scoparius | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | tigloyl-CoA is (E)-2-methylcrotonoyl-CoA | Lupinus polyphyllus x Lupinus arboreus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | isoform A and B | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? | |
tigloyl-CoA + 13-hydroxylupanine | enzyme is involved in the biosynthesis of quinolizidine alkaloid esters | Lupinus albus | CoA + 13-tigloyloxylupanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, isoform A and B, SDS-PAGE | Lupinus albus |
Synonyms | Comment | Organism |
---|---|---|
tigloyl:13alpha-hydroxylupanine O-tigloyltransferase | HLTase | Lupinus albus |
tigloyl:13alpha-hydroxymultiflorine O-tigloyltransferase | HMTase | Lupinus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
5 min, complete inactivation | Lupinus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
isoform 1 pI: 7.8, isoform 2 pI: 7.6 | Lupinus albus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
CoA | - |
Lupinus albus | |
1.7 | - |
(+)-lupanine | - |
Lupinus albus | |
3.1 | - |
(+)-epilupinine | - |
Lupinus albus |