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Literature summary for 2.3.1.94 extracted from

  • Tsai, S.C.; Miercke, L.J.W.; Krucinski, J.; Gokhale, R.; Chen, J.C.H.; Foster, P.G.; Cane, D.E.; Khosla, C.; Stroud, R.M.
    Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: Versatility from a unique substrate channel (2001), Proc. Natl. Acad. Sci. USA, 98, 14808-14813.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology polyketide synthases synthesize the polyketide cores of biologically active compounds, including natural products that have become important pharmaceuticals, like erythromycin Saccharopolyspora erythraea

Cloned(Commentary)

Cloned (Comment) Organism
the thioesterase domain from deoxyerythronolide B synthase is cloned for expression in Escherichia coli cells Saccharopolyspora erythraea

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of the thioesterase domain from deoxyerythronolide B synthase is solved to 2.8 A resolution Saccharopolyspora erythraea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
thioesterase domain, 2 * 33000 Saccharopolyspora erythraea
66000
-
thioesterase domain, homodimer Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ Saccharopolyspora erythraea
-
6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea Q03133
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-agarose and a Poros HQ column Saccharopolyspora erythraea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+
-
Saccharopolyspora erythraea 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
-
?

Subunits

Subunits Comment Organism
homodimer thioesterase domain, 2 * 33000 Saccharopolyspora erythraea
More deoxyerythronolide B synthase consists of several modules, formed by acyltransferases, acyl carrier proteins, ketosynthases, ketoreductases, dehydratases, enoylreductases and thioesterases Saccharopolyspora erythraea

Synonyms

Synonyms Comment Organism
DEBS
-
Saccharopolyspora erythraea
deoxyerythronolide B synthase
-
Saccharopolyspora erythraea
erythromycin polyketide synthase
-
Saccharopolyspora erythraea
PKS
-
Saccharopolyspora erythraea
polyketide synthase
-
Saccharopolyspora erythraea