Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.95 extracted from

  • Lim, C.; Fowler, Z.; Hueller, T.; Schaffer, S.; Koffas, M.
    High-yield resveratrol production in engineered Escherichia coli (2011), Appl. Environ. Microbiol., 77, 3451-3460.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Arachis hypogaea
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus densiflora
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Vitis vinifera
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus strobus
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Polygonum cuspidatum
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Pinus massoniana
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis Psilotum nudum

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA competitive inhibition Arachis hypogaea
acetyl-CoA
-
Pinus massoniana
acetyl-CoA competitive inhibition Pinus strobus
acetyl-CoA competitive inhibition Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00287
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
0.00443
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea
0.00856
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera
0.0119
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA Arachis hypogaea
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Pinus densiflora
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Vitis vinifera
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Pinus strobus
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Polygonum cuspidatum
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Pinus massoniana
-
4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA Psilotum nudum
-
4 CoA + trans-resveratrol + 4 CO2
-
?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
gene sts
-
Pinus densiflora
-
gene sts
-
Pinus massoniana
-
gene sts
-
Pinus strobus
-
gene sts
-
Polygonum cuspidatum
-
gene sts
-
Psilotum nudum
-
gene sts
-
Vitis vinifera
-
gene sts
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Arachis hypogaea
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus densiflora
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Vitis vinifera
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus strobus
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Polygonum cuspidatum
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Pinus massoniana
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography Psilotum nudum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA
-
Arachis hypogaea 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Pinus densiflora 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Vitis vinifera 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Pinus strobus 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Polygonum cuspidatum 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Pinus massoniana 4 CoA + trans-resveratrol + 4 CO2
-
?
3 malonyl-CoA + 4-coumaroyl-CoA
-
Psilotum nudum 4 CoA + trans-resveratrol + 4 CO2
-
?

Synonyms

Synonyms Comment Organism
stilbene synthase
-
Arachis hypogaea
stilbene synthase
-
Pinus densiflora
stilbene synthase
-
Vitis vinifera
stilbene synthase
-
Pinus strobus
stilbene synthase
-
Polygonum cuspidatum
stilbene synthase
-
Pinus massoniana
stilbene synthase
-
Psilotum nudum
STS
-
Arachis hypogaea
STS
-
Pinus densiflora
STS
-
Vitis vinifera
STS
-
Pinus strobus
STS
-
Polygonum cuspidatum
STS
-
Pinus massoniana
STS
-
Psilotum nudum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arachis hypogaea
30
-
assay at Pinus densiflora
30
-
assay at Vitis vinifera
30
-
assay at Pinus strobus
30
-
assay at Polygonum cuspidatum
30
-
assay at Pinus massoniana
30
-
assay at Psilotum nudum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
0.0017
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea
0.0023
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana
0.003
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
assay at Arachis hypogaea
4
-
assay at Pinus densiflora
4
-
assay at Vitis vinifera
4
-
assay at Pinus strobus
4
-
assay at Polygonum cuspidatum
4
-
assay at Pinus massoniana
4
-
assay at Psilotum nudum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Vitis vinifera
0.04
-
acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Pinus massoniana
0.07
-
acetyl-CoA pH 4.0, 30°C, recombinant STS, uncompetitive inhibition Arachis hypogaea
0.15
-
acetyl-CoA pH 4.0, 30°C, uncompetitive inhibition Pinus strobus
0.46
-
acetyl-CoA pH 4.0, 30°C, competitive inhibition Vitis vinifera
0.52
-
acetyl-CoA pH 4.0, 30°C, recombinant STS, competitive inhibition Arachis hypogaea
0.787
-
acetyl-CoA pH 4.0, 30°C, competitive inhibition Pinus massoniana
0.97
-
acetyl-CoA pH 4.0, 30°C, competitive inhibition Pinus strobus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.107
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus massoniana
0.16
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Pinus strobus
0.21
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Vitis vinifera
0.314
-
4-coumaroyl-CoA pH 4.0, 30°C, recombinant STS Arachis hypogaea