Cloned (Comment) | Organism |
---|---|
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Arachis hypogaea |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus densiflora |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Vitis vinifera |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus strobus |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Polygonum cuspidatum |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Pinus massoniana |
gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis | Psilotum nudum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | competitive inhibition | Arachis hypogaea | |
acetyl-CoA | - |
Pinus massoniana | |
acetyl-CoA | competitive inhibition | Pinus strobus | |
acetyl-CoA | competitive inhibition | Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00287 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
0.00443 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea | |
0.00856 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera | |
0.0119 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | Arachis hypogaea | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Pinus densiflora | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Vitis vinifera | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Pinus strobus | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Polygonum cuspidatum | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Pinus massoniana | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | Psilotum nudum | - |
4 CoA + trans-resveratrol + 4 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
gene sts | - |
Pinus densiflora | - |
gene sts | - |
Pinus massoniana | - |
gene sts | - |
Pinus strobus | - |
gene sts | - |
Polygonum cuspidatum | - |
gene sts | - |
Psilotum nudum | - |
gene sts | - |
Vitis vinifera | - |
gene sts | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Arachis hypogaea |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus densiflora |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Vitis vinifera |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus strobus |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Polygonum cuspidatum |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Pinus massoniana |
recombinant His6-tagged STS from Escherichia coli strain BL21 Star by nickel affinity chromatography | Psilotum nudum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Arachis hypogaea | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus densiflora | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Vitis vinifera | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus strobus | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Polygonum cuspidatum | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Pinus massoniana | 4 CoA + trans-resveratrol + 4 CO2 | - |
? | |
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Psilotum nudum | 4 CoA + trans-resveratrol + 4 CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
stilbene synthase | - |
Arachis hypogaea |
stilbene synthase | - |
Pinus densiflora |
stilbene synthase | - |
Vitis vinifera |
stilbene synthase | - |
Pinus strobus |
stilbene synthase | - |
Polygonum cuspidatum |
stilbene synthase | - |
Pinus massoniana |
stilbene synthase | - |
Psilotum nudum |
STS | - |
Arachis hypogaea |
STS | - |
Pinus densiflora |
STS | - |
Vitis vinifera |
STS | - |
Pinus strobus |
STS | - |
Polygonum cuspidatum |
STS | - |
Pinus massoniana |
STS | - |
Psilotum nudum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arachis hypogaea |
30 | - |
assay at | Pinus densiflora |
30 | - |
assay at | Vitis vinifera |
30 | - |
assay at | Pinus strobus |
30 | - |
assay at | Polygonum cuspidatum |
30 | - |
assay at | Pinus massoniana |
30 | - |
assay at | Psilotum nudum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
0.0017 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea | |
0.0023 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana | |
0.003 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Arachis hypogaea |
4 | - |
assay at | Pinus densiflora |
4 | - |
assay at | Vitis vinifera |
4 | - |
assay at | Pinus strobus |
4 | - |
assay at | Polygonum cuspidatum |
4 | - |
assay at | Pinus massoniana |
4 | - |
assay at | Psilotum nudum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Vitis vinifera | |
0.04 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Pinus massoniana | |
0.07 | - |
acetyl-CoA | pH 4.0, 30°C, recombinant STS, uncompetitive inhibition | Arachis hypogaea | |
0.15 | - |
acetyl-CoA | pH 4.0, 30°C, uncompetitive inhibition | Pinus strobus | |
0.46 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Vitis vinifera | |
0.52 | - |
acetyl-CoA | pH 4.0, 30°C, recombinant STS, competitive inhibition | Arachis hypogaea | |
0.787 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Pinus massoniana | |
0.97 | - |
acetyl-CoA | pH 4.0, 30°C, competitive inhibition | Pinus strobus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus massoniana | |
0.16 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Pinus strobus | |
0.21 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Vitis vinifera | |
0.314 | - |
4-coumaroyl-CoA | pH 4.0, 30°C, recombinant STS | Arachis hypogaea |