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Literature summary for 2.3.1.97 extracted from
- Identification and characterization of multiple forms of bovine brain N-myristoyltransferase (1995), J. Biol. Chem., 270, 23226-23233.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme forms multiple high molecular weight aggregates of fully active monomers | Bos taurus |
| 60000 | - |
1 * 60000, SDS-PAGE | Bos taurus |
| 126000 | 390000 | high molecular weight aggregates, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| multiple forms from brain | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, conversion of 60000 Da form into 47000 Da form, loss of 30% activity within 10 days | Bos taurus |
| 4°C, conversion of 60000 Da form into 47000 Da form, activity is stable over several months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg | pp60src-derived peptide | Bos taurus | N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-Arg + CoA | - |
? |  |
| myristoyl-CoA + glycylpeptide | specific for Gly at N-terminus | Bos taurus | N-myristoylglycylpeptide + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Bos taurus |
| monomer | 1 * 47000-49000, active form after storage at 4°C, SDS-PAGE | Bos taurus |
| More | 2 interconvertable forms of 60000 and 47000 Da, both catalytically active, formation of multimeric complexes, molecular weight reduction is not only due to proteolysis but has a possible regulatory role in vivo | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NMT | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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