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Literature summary for 2.3.1.97 extracted from

  • Pasha, M.K.; Dimmock, J.R.; Hollenberg, M.D.; Sharma, R.K.
    Enhanced activity of human N-myristoyltransferase by dimethyl sulfoxide and related solvents in the presence of serine/threonine-containing peptide substrates (2002), Biochem. Pharmacol., 64, 1461-1467.
    View publication on PubMed
Search for more literature for 2.3.1.97

Activating Compound

Activating Compound Comment Organism Structure
dimethyl ammonium chloride 6.5fold increase in activity with Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg as substrate, 2.5fold increase with Gly-Asn-Ala-Ala-Ala-Ala-Lys-Lys-Arg-Arg Homo sapiens
Dimethylsulfoxide activation mechanism Homo sapiens
Dimethylsulfoxide 8.5fold increase in activity with Gly-Ala-Gln-Phe-Ser-Lys-Thr-Ala-Arg-Arg, 10fold with Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg and 7fold increase with Gly-Asn-Ala-Ser-Ser-Ile-Lys-Lys-Lys Homo sapiens
Dimethylsulfoxide activation requires a serine in the peptide substrate Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Oxidation Stability

Oxidation Stability Organism
dimethylsulfoxide, stable up to 40% Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + cAMP-dependent protein kinase-derived peptide i.e. Gly-Asn-Ala-Ala-Ala-Ala-Lys-Lys-Arg-Arg Homo sapiens N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA
-
 ?
myristoyl-CoA + Gly-Ala-Gln-Phe-Ser-Lys-Thr-Ala-Arg-Arg i.e. myristolated alanine-rich C-kinase substrate MARCKS Homo sapiens N-myristoyl-Gly-Ala-Gln-Phe-Ser-Lys-Thr-Ala-Arg-Arg-derived peptide + CoA
-
 ?
myristoyl-CoA + Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg pp60src-derived peptide Homo sapiens N-myristoyl-Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Arg + CoA
-
 ?
myristoyl-CoA + M2 gene segment of reovirus type 3-derived peptide i.e. Gly-Asn-Ala-Ser-Ser-Ile-Lys-Lys-Lys Homo sapiens N-myristoylated M2 gene segment of reovirus type 3-derived peptide + CoA
-
 ?

Synonyms

Synonyms Comment Organism
NMT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens