Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aspergillus fumigatus |
Crystallization (Comment) | Organism |
---|---|
in complex with inhibitors 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide and 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide binding in the peptide substrate pocket | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide | inhibits the enzyme and is fungicidal under partially repressive nmt conditions | Aspergillus fumigatus | |
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide | - |
Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
myristoleoyl-CoA | pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | |
0.022 | - |
CAP5.5 | pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q9UVX3 | - |
- |
Aspergillus fumigatus ATCC MYA-4609 | Q9UVX3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoleoyl-CoA + CAP5.5 | acceptor is biotin-tagged cytoskeleton-associated protein 5.5 | Aspergillus fumigatus | N-myristoleoyl-CAP5.5 + CoA | - |
? | |
myristoleoyl-CoA + CAP5.5 | acceptor is biotin-tagged cytoskeleton-associated protein 5.5 | Aspergillus fumigatus ATCC MYA-4609 | N-myristoleoyl-CAP5.5 + CoA | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000012 | - |
pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide | |
0.000023 | - |
pH not specified in the publication, temperature not specified in the publication | Aspergillus fumigatus | 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide |
General Information | Comment | Organism |
---|---|---|
physiological function | expression of the Nmt gene is essential for viability. Partial repression of the gene reveals downstream effects of N-myristoylation on cell wall morphology | Aspergillus fumigatus |