Any feedback?
Literature summary for 2.3.1.B36 extracted from
- Cloning of glycerophosphocholine acyltransferase (GPCAT) from fungi and plants a novel enzyme phosphatidylcholine synthesis (2016), J. Biol. Chem., 291, 25066-25076 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GPC1 or At5g35460, sequence comparisons, functional recombinant expression in Saccharoymces cerevisiae | Arabidopsis thaliana |
| gene GPC1 or BnaA04g07370D, sequence comparisons, functional recombinant expression in Saccharoymces cerevisiae | Brassica napus |
| gene GPC1 or XM_002514040, sequence comparisons, functional recombinant expression in Saccharoymces cerevisiae | Ricinus communis |
| gene GPC1 or YGR149W, sequence comparisons | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | plant homologues of GPC1 catalyze acyl-CoA-dependent acylation of GPC when expressed in yeast. Identification of a deletion strain lacking GPCAT activity, but exhibiting glycerol 3-phosphate (G3P) acylation. The identified strain bears a deletion in ORF YGR149W encoding GPC1. Wild-type GPC1 complements the deletion strain | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | Gpc1p is an intergral membrane protein | Arabidopsis thaliana | 16020 | - |
| membrane | Gpc1p is an intergral membrane protein | Ricinus communis | 16020 | - |
| membrane | Gpc1p is an intergral membrane protein | Brassica napus | 16020 | - |
| membrane | Gpc1p is an intergral membrane protein with eight transmembrane helices | Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| microsome | - |
Brassica napus | - |
- |
| microsome | microsomal preparations from castor bean seeds have high GPCAT activity | Ricinus communis | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + glycerophosphocholine | Saccharomyces cerevisiae | - |
CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? |  |
| acyl-CoA + glycerophosphocholine | Arabidopsis thaliana | - |
CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | Ricinus communis | - |
CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | Brassica napus | - |
CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | Saccharomyces cerevisiae ATCC 204508 | - |
CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | Arabidopsis thaliana | - |
CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | Brassica napus | - |
CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FJB4 | - |
- |
| Brassica napus | A0A078H868 | - |
- |
| Ricinus communis | B9RK42 | - |
- |
| Saccharomyces cerevisiae | P48236 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | P48236 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.032 | - |
pH 7.2, 30°C, with substrates palmitoyl-CoA and glycerophosphocholine | Brassica napus |
| 0.038 | - |
about, pH 7.2, 30°C, with substrates palmitoyl-CoA and glycerophosphocholine | Ricinus communis |
| 0.052 | - |
pH 7.2, 30°C, with substrates palmitoyl-CoA and glycerophosphocholine | Arabidopsis thaliana |
| 0.086 | - |
pH 7.2, 30°C, with substrates palmitoyl-CoA and glycerophosphocholine | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-oleoyl-sn-glycero-3-phosphatidic acid + glycerophosphocholine | very low activity compared to lysphosphatidylcholine | Saccharomyces cerevisiae | glycero-3-phosphatidic acid + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| 1-oleoyl-sn-glycero-3-phosphocholine + glycerophosphocholine | best lysolipid substrate | Saccharomyces cerevisiae | glycero-3-phosphocholine + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| 1-oleoyl-sn-glycero-3-phosphoethanolamine + glycerophosphocholine | moderate activity compared to lysphosphatidylcholine | Saccharomyces cerevisiae | glycero-3-phosphoethanolamine + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| 1-oleoyl-sn-glycero-3-phosphoserine + glycerophosphocholine | low activity compared to lysphosphatidylcholine | Saccharomyces cerevisiae | glycero-3-phosphoserine + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | - |
Saccharomyces cerevisiae | CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | - |
Arabidopsis thaliana | CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | - |
Ricinus communis | CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | - |
Brassica napus | CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| acyl-CoA + glycerophosphocholine | - |
Saccharomyces cerevisiae ATCC 204508 | CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
? | |
| linolenoyl-CoA + glycerophosphocholine | high activity | Saccharomyces cerevisiae | CoA + 1-linolenoyl-sn-glycero-3-phosphocholine | - |
? | |
| linolenoyl-CoA + glycerophosphocholine | high activity | Ricinus communis | CoA + 1-linolenoyl-sn-glycero-3-phosphocholine | - |
? | |
| linolenoyl-CoA + glycerophosphocholine | high activity | Saccharomyces cerevisiae ATCC 204508 | CoA + 1-linolenoyl-sn-glycero-3-phosphocholine | - |
? | |
| linoleoyl-CoA + glycerophosphocholine | high activity | Saccharomyces cerevisiae | CoA + 1-linoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| linoleoyl-CoA + glycerophosphocholine | high activity | Ricinus communis | CoA + 1-linoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| linoleoyl-CoA + glycerophosphocholine | high activity | Saccharomyces cerevisiae ATCC 204508 | CoA + 1-linoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| additional information | acyl-CoA substrate specificty of GCP1p with glycerophosphocholine, overview | Ricinus communis | ? | - |
- |
|
| additional information | lysolipids can also serve as acyl donor substrates for ScGPCAT (Gpc1p) activity. ScGPCAT acyl-CoA substrate specificity and transacylation activity of GPCAT, overview | Saccharomyces cerevisiae | ? | - |
- |
|
| additional information | lysolipids can also serve as acyl donor substrates for ScGPCAT (Gpc1p) activity. ScGPCAT acyl-CoA substrate specificity and transacylation activity of GPCAT, overview | Saccharomyces cerevisiae ATCC 204508 | ? | - |
- |
|
| oleoyl-CoA + glycerophosphocholine | high activity | Ricinus communis | CoA + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| oleoyl-CoA + glycerophosphocholine | moderate activity | Saccharomyces cerevisiae | CoA + 1-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| oleoyl-CoA + glycerophosphoethanolamine | enzyme Gpc1p also catalyzes the acylation of GPE with acyl-CoA, with about one third of activity compared to glycerophosphocholine | Saccharomyces cerevisiae | CoA + 1-oleoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
| palmitoleoyl-CoA + glycerophosphocholine | moderate activity | Saccharomyces cerevisiae | CoA + 1-palmitoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoleoyl-CoA + glycerophosphocholine | moderate activity | Ricinus communis | CoA + 1-palmitoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | - |
Arabidopsis thaliana | CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | - |
Brassica napus | CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | high activity, best substrate | Saccharomyces cerevisiae | CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | high activity, best substrate | Ricinus communis | CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| palmitoyl-CoA + glycerophosphocholine | high activity, best substrate | Saccharomyces cerevisiae ATCC 204508 | CoA + 1-palmitoyl-sn-glycero-3-phosphocholine | - |
? | |
| ricinoleoyl-CoA + glycerophosphocholine | high activity | Saccharomyces cerevisiae | CoA + 1-ricinoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| ricinoleoyl-CoA + glycerophosphocholine | moderate activity | Ricinus communis | CoA + 1-ricinoleoyl-sn-glycero-3-phosphocholine | - |
? | |
| stearoyl-CoA + glycerophosphocholine | moderate activity | Saccharomyces cerevisiae | CoA + 1-stearoyl-sn-glycero-3-phosphocholine | - |
? | |
| stearoyl-CoA + glycerophosphocholine | moderate activity | Ricinus communis | CoA + 1-stearoyl-sn-glycero-3-phosphocholine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtGPCAT | - |
Arabidopsis thaliana |
| BnGPCAT | - |
Brassica napus |
| glycerophosphocholine acyltransferase | - |
Saccharomyces cerevisiae |
| glycerophosphocholine acyltransferase | - |
Arabidopsis thaliana |
| glycerophosphocholine acyltransferase | - |
Ricinus communis |
| glycerophosphocholine acyltransferase | - |
Brassica napus |
| glycerophosphocholine acyltransferase 1 | UniProt | Saccharomyces cerevisiae |
| glycerophosphocholine acyltransferase 1 | UniProt | Arabidopsis thaliana |
| glycerophosphocholine acyltransferase 1 | UniProt | Ricinus communis |
| glycerophosphocholine acyltransferase 1 | UniProt | Brassica napus |
| GPC1 | gene name, UniProt | Saccharomyces cerevisiae |
| GPC1 | gene name, UniProt | Arabidopsis thaliana |
| GPC1 | gene name, UniProt | Ricinus communis |
| GPC1 | gene name, UniProt | Brassica napus |
| Gpc1p | - |
Saccharomyces cerevisiae |
| Gpc1p | - |
Arabidopsis thaliana |
| Gpc1p | - |
Ricinus communis |
| Gpc1p | - |
Brassica napus |
| GPCAT | - |
Saccharomyces cerevisiae |
| GPCAT | - |
Arabidopsis thaliana |
| GPCAT | - |
Ricinus communis |
| GPCAT | - |
Brassica napus |
| RcGPCAT | - |
Ricinus communis |
| ScGPCAT | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
assay at | Arabidopsis thaliana |
| 30 | - |
assay at | Ricinus communis |
| 30 | - |
assay at | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Saccharomyces cerevisiae |
| 7.2 | - |
assay at | Arabidopsis thaliana |
| 7.2 | - |
assay at | Ricinus communis |
| 7.2 | - |
assay at | Brassica napus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | evolution of the GPCAT family in eukaryotes, phylogenetic tree, evolutionary analysis, overview. GPCAT does not show any significant homology to other known acyl transferases and constitutes an enzyme family on its own with homologues represented in major eukaryotic organism groups but absent in prokaryotes | Saccharomyces cerevisiae |
| evolution | evolution of the GPCAT family in eukaryotes, phylogenetic tree, evolutionary analysis, overview. GPCAT does not show any significant homology to other known acyl transferases and constitutes an enzyme family on its own with homologues represented in major eukaryotic organism groups but absent in prokaryotes | Arabidopsis thaliana |
| evolution | evolution of the GPCAT family in eukaryotes, phylogenetic tree, evolutionary analysis, overview. GPCAT does not show any significant homology to other known acyl transferases and constitutes an enzyme family on its own with homologues represented in major eukaryotic organism groups but absent in prokaryotes | Ricinus communis |
| evolution | evolution of the GPCAT family in eukaryotes, phylogenetic tree, evolutionary analysis, overview. GPCAT does not show any significant homology to other known acyl transferases and constitutes an enzyme family on its own with homologues represented in major eukaryotic organism groups but absent in prokaryotes | Brassica napus |
| physiological function | cellular role for Gpc1p in the synthesis of phosphocholine via acylation of GPC. GPCAT is also unique in that it has, in addition to acyl-CoA acyltransferase activity, LPC:GPC transacylase activity | Saccharomyces cerevisiae |
| physiological function | cellular role for Gpc1p in the synthesis of phosphocholine via acylation of GPC. GPCAT is also unique in that it has, in addition to acyl-CoA acyltransferase activity, LPC:GPC transacylase activity | Arabidopsis thaliana |
| physiological function | cellular role for Gpc1p in the synthesis of phosphocholine via acylation of GPC. GPCAT is also unique in that it has, in addition to acyl-CoA acyltransferase activity, LPC:GPC transacylase activity | Ricinus communis |
| physiological function | cellular role for Gpc1p in the synthesis of phosphocholine via acylation of GPC. GPCAT is also unique in that it has, in addition to acyl-CoA acyltransferase activity, LPC:GPC transacylase activity | Brassica napus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
