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Literature summary for 2.3.1.B37 extracted from

  • Takenaka, S.; Yoshida, K.; Tanaka, K.; Yoshida, K.
    Molecular characterization of a novel N-acetyltransferase from Chryseobacterium sp. (2014), Appl. Environ. Microbiol., 80, 1770-1776.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chryseobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 35.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
AgNO3 22.4% residual activity after 1 h at 1 mM Chryseobacterium sp.
Cu2+ 79.5% residual activity after 1 h at 1 mM Chryseobacterium sp.
diethyldicarbonate 49.3% residual activity after 1 h at 1 mM Chryseobacterium sp.
diethylpyrocarbonate 35.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
Hg2+ 19.8% residual activity after 1 h at 1 mM Chryseobacterium sp.
Mg2+ 94.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
additional information Mn2+, EDTA, tiron, alpha,alpha'-dipyridyl, phenylglyoxal, phenylmethylsulfonyl fluoride, iodoacetic acid and iodoacetamide do not markedly inhibit the enzyme Chryseobacterium sp.
N-ethylmaleimide 34.5% residual activity after 1 h at 1 mM Chryseobacterium sp.
o-phenanthroline 93.1% residual activity after 1 h at 1 mM Chryseobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 L-2-phenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
0.77
-
 L-2-phenylglycine methyl ester at pH 7.5 and 30°C Chryseobacterium sp.
1.41
-
 L-2-chlorophenylglycine at pH 7.5 and 30°C Chryseobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 18000, SDS-PAGE Chryseobacterium sp.

Organism

Organism UniProt Comment Textmining
Chryseobacterium sp. W0SLW8
-
-
Chryseobacterium sp. 5-3B W0SLW8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and Cosmogel His-accept column chromatography Chryseobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
 cell extract, at pH 7.5 and 30°C Chryseobacterium sp.
10.5
-
 purified enzyme, at pH 7.5 and 30°C Chryseobacterium sp.

Storage Stability

Storage Stability Organism
4°C, 50 mM sodium potassium phosphate buffer (pH 7.5) containing 0.3 M NaCl, more than 1 month, no loss of activity Chryseobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + (+/-)-1-phenylethylamine 6.3% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
acetyl-CoA + (+/-)-1-phenylethylamine 6.3% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
acetyl-CoA + DL-2-chlorophenylglycine 56.9% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
acetyl-CoA + DL-2-chlorophenylglycine 56.9% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
acetyl-CoA + DL-2-phenylglycine 98% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
acetyl-CoA + DL-2-phenylglycine 98% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
acetyl-CoA + DL-4-chlorophenylglycine 7.4% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
acetyl-CoA + DL-4-chlorophenylglycine 7.4% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
acetyl-CoA + L-2-chlorophenylglycine 52.9% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-2-chlorophenylglycine
-
 ?
acetyl-CoA + L-2-phenylglycine 100% activity Chryseobacterium sp. CoA + (2S)-2-acetylamino-2-phenylacetic acid
-
 ?
acetyl-CoA + L-2-phenylglycine methyl ester 75.5% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-2-phenylglycine methyl ester
-
 ?
acetyl-CoA + L-4-hydroxyphenylglycine 16.7% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-4-hydroxyphenylglycine
-
 ?
additional information the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) Chryseobacterium sp. ?
-
 ?
additional information the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) Chryseobacterium sp. 5-3B ?
-
 ?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Chryseobacterium sp.

Synonyms

Synonyms Comment Organism
NatA
-
 Chryseobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Chryseobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.31
-
 L-2-chlorophenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
13.6
-
 L-2-phenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
19.2
-
 L-2-phenylglycine methyl ester at pH 7.5 and 30°C Chryseobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Chryseobacterium sp.