Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Chryseobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 35.2% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
AgNO3 | 22.4% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
Cu2+ | 79.5% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
diethyldicarbonate | 49.3% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
diethylpyrocarbonate | 35.2% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
Hg2+ | 19.8% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
Mg2+ | 94.2% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
additional information | Mn2+, EDTA, tiron, alpha,alpha'-dipyridyl, phenylglyoxal, phenylmethylsulfonyl fluoride, iodoacetic acid and iodoacetamide do not markedly inhibit the enzyme | Chryseobacterium sp. | |
N-ethylmaleimide | 34.5% residual activity after 1 h at 1 mM | Chryseobacterium sp. | |
o-phenanthroline | 93.1% residual activity after 1 h at 1 mM | Chryseobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.145 | - |
L-2-phenylglycine | at pH 7.5 and 30°C | Chryseobacterium sp. | |
0.77 | - |
L-2-phenylglycine methyl ester | at pH 7.5 and 30°C | Chryseobacterium sp. | |
1.41 | - |
L-2-chlorophenylglycine | at pH 7.5 and 30°C | Chryseobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, SDS-PAGE | Chryseobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chryseobacterium sp. | W0SLW8 | - |
- |
Chryseobacterium sp. 5-3B | W0SLW8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and Cosmogel His-accept column chromatography | Chryseobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
cell extract, at pH 7.5 and 30°C | Chryseobacterium sp. |
10.5 | - |
purified enzyme, at pH 7.5 and 30°C | Chryseobacterium sp. |
Storage Stability | Organism |
---|---|
4°C, 50 mM sodium potassium phosphate buffer (pH 7.5) containing 0.3 M NaCl, more than 1 month, no loss of activity | Chryseobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (+/-)-1-phenylethylamine | 6.3% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + ? | - |
? | |
acetyl-CoA + (+/-)-1-phenylethylamine | 6.3% activity compared to L-2-phenylglycine | Chryseobacterium sp. 5-3B | CoA + ? | - |
? | |
acetyl-CoA + DL-2-chlorophenylglycine | 56.9% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + ? | - |
? | |
acetyl-CoA + DL-2-chlorophenylglycine | 56.9% activity compared to L-2-phenylglycine | Chryseobacterium sp. 5-3B | CoA + ? | - |
? | |
acetyl-CoA + DL-2-phenylglycine | 98% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + ? | - |
? | |
acetyl-CoA + DL-2-phenylglycine | 98% activity compared to L-2-phenylglycine | Chryseobacterium sp. 5-3B | CoA + ? | - |
? | |
acetyl-CoA + DL-4-chlorophenylglycine | 7.4% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + ? | - |
? | |
acetyl-CoA + DL-4-chlorophenylglycine | 7.4% activity compared to L-2-phenylglycine | Chryseobacterium sp. 5-3B | CoA + ? | - |
? | |
acetyl-CoA + L-2-chlorophenylglycine | 52.9% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + N-acetyl-L-2-chlorophenylglycine | - |
? | |
acetyl-CoA + L-2-phenylglycine | 100% activity | Chryseobacterium sp. | CoA + (2S)-2-acetylamino-2-phenylacetic acid | - |
? | |
acetyl-CoA + L-2-phenylglycine methyl ester | 75.5% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + N-acetyl-L-2-phenylglycine methyl ester | - |
? | |
acetyl-CoA + L-4-hydroxyphenylglycine | 16.7% activity compared to L-2-phenylglycine | Chryseobacterium sp. | CoA + N-acetyl-L-4-hydroxyphenylglycine | - |
? | |
additional information | the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) | Chryseobacterium sp. | ? | - |
? | |
additional information | the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) | Chryseobacterium sp. 5-3B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE | Chryseobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
NatA | - |
Chryseobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Chryseobacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.31 | - |
L-2-chlorophenylglycine | at pH 7.5 and 30°C | Chryseobacterium sp. | |
13.6 | - |
L-2-phenylglycine | at pH 7.5 and 30°C | Chryseobacterium sp. | |
19.2 | - |
L-2-phenylglycine methyl ester | at pH 7.5 and 30°C | Chryseobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Chryseobacterium sp. |