Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Penicillium brefeldianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 malonyl-CoA + 4 NADPH + 16 H+ | Penicillium brefeldianum | - |
8 CoA + (2E,4E,10E)-15-hydroxyhexadeca-2,4,10-trienoic acid + 8 CO2 + 4 NADP+ + 6 H2O | main product in presence of thiohydrolase Bref-TH | ? | |
8 malonyl-CoA + 4 NADPH + 16 H+ | Penicillium brefeldianum ATCC 58665 | - |
8 CoA + (2E,4E,10E)-15-hydroxyhexadeca-2,4,10-trienoic acid + 8 CO2 + 4 NADP+ + 6 H2O | main product in presence of thiohydrolase Bref-TH | ? | |
9 malonyl-CoA + 5 NADPH + 19 H+ | Penicillium brefeldianum | - |
9 CoA + (4E,6Z,12E)-17-hydroxyoctadeca-4,6,12-trienoic acid + 9 CO2 + 5 NADP+ + 7 H2O | main product in absence of thiohydrolase Bref-TH | ? | |
9 malonyl-CoA + 5 NADPH + 19 H+ | Penicillium brefeldianum ATCC 58665 | - |
9 CoA + (4E,6Z,12E)-17-hydroxyoctadeca-4,6,12-trienoic acid + 9 CO2 + 5 NADP+ + 7 H2O | main product in absence of thiohydrolase Bref-TH | ? | |
additional information | Penicillium brefeldianum | enzyme shows an NADPH-dependent reductive tailoring specificity that leads to the synthesis of four different oktaketide products with varying degrees of reduction. Bref-PKS is a nonaketide synthase in the absence of an associated thiohydrolase Bref-TH | ? | - |
? | |
additional information | Penicillium brefeldianum ATCC 58665 | enzyme shows an NADPH-dependent reductive tailoring specificity that leads to the synthesis of four different oktaketide products with varying degrees of reduction. Bref-PKS is a nonaketide synthase in the absence of an associated thiohydrolase Bref-TH | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium brefeldianum | - |
- |
- |
Penicillium brefeldianum ATCC 58665 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8 malonyl-CoA + 4 NADPH + 16 H+ | - |
Penicillium brefeldianum | 8 CoA + (2E,4E,10E)-15-hydroxyhexadeca-2,4,10-trienoic acid + 8 CO2 + 4 NADP+ + 6 H2O | main product in presence of thiohydrolase Bref-TH | ? | |
8 malonyl-CoA + 4 NADPH + 16 H+ | - |
Penicillium brefeldianum ATCC 58665 | 8 CoA + (2E,4E,10E)-15-hydroxyhexadeca-2,4,10-trienoic acid + 8 CO2 + 4 NADP+ + 6 H2O | main product in presence of thiohydrolase Bref-TH | ? | |
9 malonyl-CoA + 5 NADPH + 19 H+ | - |
Penicillium brefeldianum | 9 CoA + (4E,6Z,12E)-17-hydroxyoctadeca-4,6,12-trienoic acid + 9 CO2 + 5 NADP+ + 7 H2O | main product in absence of thiohydrolase Bref-TH | ? | |
9 malonyl-CoA + 5 NADPH + 19 H+ | - |
Penicillium brefeldianum ATCC 58665 | 9 CoA + (4E,6Z,12E)-17-hydroxyoctadeca-4,6,12-trienoic acid + 9 CO2 + 5 NADP+ + 7 H2O | main product in absence of thiohydrolase Bref-TH | ? | |
additional information | enzyme shows an NADPH-dependent reductive tailoring specificity that leads to the synthesis of four different oktaketide products with varying degrees of reduction. Bref-PKS is a nonaketide synthase in the absence of an associated thiohydrolase Bref-TH | Penicillium brefeldianum | ? | - |
? | |
additional information | enzyme shows an NADPH-dependent reductive tailoring specificity that leads to the synthesis of four different oktaketide products with varying degrees of reduction. Bref-PKS is a nonaketide synthase in the absence of an associated thiohydrolase Bref-TH | Penicillium brefeldianum ATCC 58665 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bref-PKS | enzyme is involved in biosynthesis of brefeldin A | Penicillium brefeldianum |
highly reducing polyketide synthase | - |
Penicillium brefeldianum |
HRPKS | - |
Penicillium brefeldianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | in the absence of the reducing cofactor NADPH, the reductive domains are inactive and yield an unreduced polyketide product | Penicillium brefeldianum |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is involved in biosynthesis of brefeldin A. It synthesizes the precisely reduced oktaketide precursor, which can then be directly offloaded by the thiohydrolase enzyme followed by a P450-mediated formation of the cyclopentane ring and macrocyclization to afford 7-deoxy brefeldin A.thiolase Bref-TH controls the programmed release of the oktaketide product from synthase Bref-PKS | Penicillium brefeldianum |