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Literature summary for 2.3.2.10 extracted from

  • Maillard, A.P.; Biarrotte-Sorin, S.; Villet, R.; Mesnage, S.; Bouhss, A.; Sougakoff, W.; Mayer, C.; Arthur, M.
    Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens (2005), J. Bacteriol., 187, 3833-3838.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.10

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli TOP10 Weissella viridescens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine by vapor diffusion method Weissella viridescens

Protein Variants

Protein Variants Comment Organism
F70L 35% loss of activity Weissella viridescens
K36M activity complete abolished Weissella viridescens
K36R 160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens
R211K 17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration Weissella viridescens
Y103F no effect on activity Weissella viridescens
Y215F 25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens
Y215L insoluble protein Weissella viridescens
Y256F 2.6-fold reduction of activity Weissella viridescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 L-alanyl-tRNA pH 7.5, 30°C Weissella viridescens
0.079
-
 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine pH 7.5, 30°C Weissella viridescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens production of precursors for branched chain peptidoglycans tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens CIP 102810 / ATCC 12706 production of precursors for branched chain peptidoglycans tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Weissella viridescens
-
 formerly Lactobacillus viridescens
-
Weissella viridescens CIP 102810 / ATCC 12706
-
 formerly Lactobacillus viridescens
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Weissella viridescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine production of precursors for branched chain peptidoglycans Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens CIP 102810 / ATCC 12706 tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine production of precursors for branched chain peptidoglycans Weissella viridescens CIP 102810 / ATCC 12706 tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
additional information enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide Weissella viridescens ?
-
 ?
additional information enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide Weissella viridescens CIP 102810 / ATCC 12706 ?
-
 ?

Synonyms

Synonyms Comment Organism
FemX
-
 Weissella viridescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.7
-
 L-alanyl-tRNA pH 7.5, 30°C Weissella viridescens