Literature summary for 2.3.2.12 extracted from

Dresios, J.; Panopoulos, P.; Frantziou, C.P.; Synetos, D.
Yeast Ribosomal Protein Deletion Mutants Possess Altered Peptidyltransferase Activity and Different Sensitivity to Cycloheximide (2001), Biochemistry, 40, 8101-8108.

Search for more literature for 2.3.2.12

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	wild type strain 2D-J809, strain 2A-J809 lacking the two genes encoding L24, strain L1726 lacking the single gene encoding L39, strain L1725 lacking the genes for both proteins L24 and L39	-

Storage Stability

Storage Stability	Organism
-70°C, both ribosomes and factors washable from ribosomes	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
AcPhe-tRNA + puromycin	-	Saccharomyces cerevisiae	tRNA + AcPhe-puromycin	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	enzyme assay, disk reaction and reaction in solution	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0115	-	AcPhe-tRNA	strain 2A-J809 lacking the two genes encoding L24	Saccharomyces cerevisiae
0.0178	-	AcPhe-tRNA	triple mutant strain L1725 lacking the proteins L24 and L39	Saccharomyces cerevisiae
0.0363	-	AcPhe-tRNA	wild type strain 2D-J809	Saccharomyces cerevisiae
0.0508	-	AcPhe-tRNA	strain L1729 lacking the single gene encoding L39	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)