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Literature summary for 2.3.2.13 extracted from

  • Carvajal-Vallejos, P.K.; Campos, A.; Fuentes-Prior, P.; Villalobos, E.; Almeida, A.M.; Barbera, E.; Torne, J.M.; Santos, M.
    Purification and in vitro refolding of maize chloroplast transglutaminase over-expressed in Escherichia coli (2007), Biotechnol. Lett., 29, 1255-1262.
    View publication on PubMed
Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
sub-cloned into a pET28 vector and overexpressed in Escherichia coli BL21. The recombinant proteins are present mainly as insoluble inclusion bodies. It can be effectively renatured in vitro and exhibits general TG enzyme characteristics Zea mays

Protein Variants

Protein Variants Comment Organism
production overexpression of enzyme in Escherichia coli and purification from inclusion bodies, refolding by rapid dilution in a Ca2+- and guanidine-containing buffer. Purified enzyme has similar characteristics as native protein Zea mays

Inhibitors

Inhibitors Comment Organism Structure
Boc-DON-Gln-Ile-Val-OMe
-
 Zea mays
GTP
-
 Zea mays
monodansyl cadaverine
-
 Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Zea mays 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-
Zea mays
-
 patent WWO03102128, expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zea mays
overexpression of enzyme in Escherichia coli and purification from inclusion bodies, refolding by rapid dilution in a Ca2+- and guanidine-containing buffer Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylcasein + putrescine
-
 Zea mays ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
TGZ
-
 Zea mays