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Literature summary for 2.3.2.13 extracted from
- Purification and characterization of transglutaminase from a newly isolated Streptomyces hygroscopicus (2007), Food Chem., 105, 612-618.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | enzyme activity is slightly elevated at a lower concentration of ethanol at 25°C. With increasing ethanol concentration, the enzyme activity decreases, and in the presence of 50% ethanol the enzyme activity is almost lost after incubation for 30 min at 25°C | Streptomyces hygroscopicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis (2-nitrobenzoic acid) | - |
Streptomyces hygroscopicus | |
| Cu2+ | 5 mM CuSO4, 91% inhibition | Streptomyces hygroscopicus | |
| Hg2+ | 5 mM HgCl2, 94% inhibition | Streptomyces hygroscopicus | |
| additional information | the enzyme is not inhibited by ethylenediaminetetraacetic acid, Na+, K+, Ca2+, Mg2+, Ba2+, Mn2+ and Co2+ | Streptomyces hygroscopicus | |
| Pb2+ | 5 mM Pb(CH3COO)2, 80% inhibition | Streptomyces hygroscopicus | |
| Zn2+ | 5 mM ZnSO4, 95% inhibition | Streptomyces hygroscopicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05469 | - |
N-carboxybenzoyl-L-glutaminyl-glycine | pH 6.0, 37°C | Streptomyces hygroscopicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Streptomyces hygroscopicus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | stability is strongly affected by ethanol concentration | Streptomyces hygroscopicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus | - |
WSH03-13 | - |
| Streptomyces hygroscopicus WSH03-13 | - |
WSH03-13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces hygroscopicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Streptomyces hygroscopicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Streptomyces hygroscopicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carboxybenzoyl-L-glutaminyl-glycine + hydroxylamine | - |
Streptomyces hygroscopicus | hydroxamate + ? | - |
? | |
| N-carboxybenzoyl-L-glutaminyl-glycine + hydroxylamine | - |
Streptomyces hygroscopicus WSH03-13 | hydroxamate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Streptomyces hygroscopicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGase | - |
Streptomyces hygroscopicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 45 | at pH 6.0, hydroxamate formation from N-carboxybenzoyl-L-glutaminyl-glycine and hydroxylamine | Streptomyces hygroscopicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | at pH 6.0, 30°C: about 70% of maximal activity, 50°C: about 70% of maximal activity | Streptomyces hygroscopicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
30 min, pH 6.0, about 10% loss of activity | Streptomyces hygroscopicus |
| 40 | - |
30 min, pH 6.0, about 20% loss of activity | Streptomyces hygroscopicus |
| 50 | - |
pH 6.0, 30 min, about 45% loss of activity | Streptomyces hygroscopicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | hydroxamate formation from N-carboxybenzoyl-L-glutaminyl-glycine and hydroxylamine | Streptomyces hygroscopicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 80% of maximal activity, pH 8.0: about 80% of maximal activity | Streptomyces hygroscopicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
30 min, 50% loss of activity | Streptomyces hygroscopicus |
| 5 | 8 | 30 min, stable at 10°C | Streptomyces hygroscopicus |
| 9 | - |
30 min, 20% loss of activity | Streptomyces hygroscopicus |
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