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Literature summary for 2.3.2.13 extracted from
- Partial purification and characterization of transglutaminase from threadfin bream (Nemipterus sp.) liver (2008), J. Food Biochem., 32, 182-200.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Nemipterus sp. |  |
| Co2+ | - |
Nemipterus sp. | |
| Cu2+ | - |
Nemipterus sp. | |
| EDTA | - |
Nemipterus sp. | |
| Fe2+ | - |
Nemipterus sp. | |
| Mn2+ | - |
Nemipterus sp. | |
| Monoiodoacetic acid | - |
Nemipterus sp. | |
| N-ethylmaleimide | - |
Nemipterus sp. | |
| NaCl | 1.2 M, 25% inhibition | Nemipterus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enzyme requires Ca2+ up to 1 mM for full activation | Nemipterus sp. | |
| Sr2+ | 10 mM Sr2+, activates | Nemipterus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
x * 95000, SDS-PAGE | Nemipterus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nemipterus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Nemipterus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Nemipterus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.92 | - |
- |
Nemipterus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| monodansylcadaverine + N,N'-dimethylated casein | - |
Nemipterus sp. | ? | - |
? | |
| additional information | enzyme catalyzes the cross-linking of the myosin heavy chains of Nemipterus sp. | Nemipterus sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000, SDS-PAGE | Nemipterus sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGase | - |
Nemipterus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Nemipterus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | 40°C: about 50% of maximal activity, 60°C: about 90% of maximal activity | Nemipterus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Nemipterus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | pH 6.5: about 65% of maximal activity, pH 10.0: about 60% of maximal activity | Nemipterus sp. |
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