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Literature summary for 2.3.2.13 extracted from

  • Kiraly, R.; Csosz, E.; Kurtan, T.; Antus, S.; Szigeti, K.; Simon-Vecsei, Z.; Korponay-Szabo, I.R.; Keresztessy, Z.; Fesues, L.
    Functional significance of five noncanonical Ca2+-binding sites of human transglutaminase 2 characterized by site-directed mutagenesis (2009), FEBS J., 276, 7083-7096.
    View publication on PubMed

Application

Application Comment Organism
medicine TG2 is the major autoantigen in celiac disease, and testing the reactivity of mutants with autoantibodies from celiac disease patients reveals that calcium binding site S4, i.e. residues Y149 to Y159, strongly determines antigenicity Homo sapiens

Protein Variants

Protein Variants Comment Organism
C277S mutation of active site. Mutant still binds 6 Ca2+ Homo sapiens
D151N/E153Q/E154Q/E155Q/E158Q mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity. GTPase activity is activated by presence of Ca2+ Homo sapiens
D306N/N308S/N310S mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity Homo sapiens
D434N/E435Q/E437N mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity. GTPase activity is activated by presence of Ca2+ Homo sapiens
E396Q/N398S/D400N mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity Homo sapiens
E447Q/E451Q/E452Q/E454Q mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity Homo sapiens
N229S/N231S/D232N/D233N mutation of calcium binding site, does not cause major structural alterations. Mutant binds less than 6 Ca2+ and is deficient in transglutaminase activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ wild-type binds 6 Ca2+ per monomer. GTPase activity is inhibited by Ca2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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