Cloned (Comment) | Organism |
---|---|
expressed in HEK-293T/17, NIH-3T3, CHO-K1, and opossum kidney cells | Cavia porcellus |
Protein Variants | Comment | Organism |
---|---|---|
C277S | the mutant binds to heparin with about wild type affinity | Cavia porcellus |
D94A/D97A | the mutant is found at comparable levels on the cell surface with that shown for the wild type enzyme | Cavia porcellus |
K205A/R209A | the mutant shows increased activity in NIH 3T3 and HEK-293/T17 cell lysates compared to the wild type enzyme | Cavia porcellus |
K60A/R601A/K602A | the mutant shows reduced activity in NIH 3T3 cell lysates and increased activity in HEK-293/T17 cell lysates compared to the wild type enzyme | Cavia porcellus |
additional information | the TG2DELTA1-15 mutant is not detectable within the extracellular matrix | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
R281 | irreversible inhibitor | Cavia porcellus | |
R283 | irreversible inhibitor | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | heparin sulfate binding is required for extracellular matrix localization of the enzyme | Cavia porcellus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Cavia porcellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin-bound gamma-glutamine + alkylamine | Cavia porcellus | - |
fibronectin N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | Q7M0F8 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin Sepharose column chromatography | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin-bound gamma-glutamine + alkylamine | - |
Cavia porcellus | fibronectin N5-alkylglutamine + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TG2 | - |
Cavia porcellus |
tissue transglutaminase | - |
Cavia porcellus |