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Literature summary for 2.3.2.13 extracted from

  • Du, K.; Liu, Z.; Cui, W.; Zhou, L.; Liu, Y.; Du, G.; Chen, J.; Zhou, Z.
    pH-dependent activation of Streptomyces hygroscopicus transglutaminase mediated by intein (2014), Appl. Environ. Microbiol., 80, 723-729.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
68.3
-
 N-carboxybenzoyl-L-glutaminyl-glycine native enzyme, at pH 8.0 and 37°C Streptomyces hygroscopicus
69.4
-
 N-carboxybenzoyl-L-glutaminyl-glycine recombinant enzyme, at pH 8.0 and 37°C Streptomyces hygroscopicus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Streptomyces hygroscopicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-L-glutamine + alkylamine Streptomyces hygroscopicus
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?
[protein]-L-glutamine + alkylamine Streptomyces hygroscopicus WSH03-13
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-
Streptomyces hygroscopicus WSH03-13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Resource Q column chromatography Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carboxybenzoyl-L-glutaminyl-glycine + ?
-
 Streptomyces hygroscopicus L-glutamic acid gamma-monohydroxamate + ?
-
 ?
N-carboxybenzoyl-L-glutaminyl-glycine + ?
-
 Streptomyces hygroscopicus WSH03-13 L-glutamic acid gamma-monohydroxamate + ?
-
 ?
[protein]-L-glutamine + alkylamine
-
 Streptomyces hygroscopicus [protein]-N5-alkyl-L-glutamine + NH3
-
 ?
[protein]-L-glutamine + alkylamine
-
 Streptomyces hygroscopicus WSH03-13 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
microbial transglutaminase
-
 Streptomyces hygroscopicus
MTG
-
 Streptomyces hygroscopicus
TGase
-
 Streptomyces hygroscopicus