Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | - |
Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
Cd2+-bis-glutathionate | in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication | Euglena gracilis | |
0.0016 | - |
Zn2+-bis-glutathionate | in the presence of 0.02 mM GSH, at pH 8.0, temperature not specified in the publication | Euglena gracilis | |
0.0018 | - |
Zn2+-bis-glutathionate | in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication | Euglena gracilis | |
0.0025 | - |
Cd2+-bis-glutathionate | in the presence of 0.04 mM GSH, at pH 8.0, temperature not specified in the publication | Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | required | Euglena gracilis | |
Zn2+ | required | Euglena gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | A0A075TCK5 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin column chromatography, and gel filtration | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cd2+-bis-glutathionate + [Glu(-Cys)]n-Gly | - |
Euglena gracilis | ? | - |
? | |
metal-bis-glutathionate + [Glu(-Cys)]n-Gly | Zn2+-bis-glutathionate is the best cosubstrate. The order of preference for the Me-GS2 substrate is Zn2+ > Cd2+ > Mn2+ > As5+ > As3+ > Cu2+ > Ag+ | Euglena gracilis | ? | - |
? | |
additional information | the enzyme does not use gamma-glutamylcysteine or phytochelatin2 as substrate | Euglena gracilis | ? | - |
? | |
Zn2+-bis-glutathionate + [Glu(-Cys)]n-Gly | Zn2+-bis-glutathionate is the best cosubstrate | Euglena gracilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
PCS | - |
Euglena gracilis |
phytochelatin synthase | - |
Euglena gracilis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme confers resistance on Cd2+ stress | Euglena gracilis |