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Literature summary for 2.3.2.2 extracted from

  • Horiuchi, S.; Inoue, M.; Morino, Y.
    Latent active site in rat-kidney gamma-glutamyl transpeptidase. The refolding process of the large subunit and characterization of the renatured enzyme (1980), Eur. J. Biochem., 105, 93-102.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine inactivation of kidney enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study of renatured large subunit Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
1 * 48000 + 1 * 20000, about, gel filtration of denatured and native enzyme Rattus norvegicus
48000
-
1 * 48000 + 1 * 20000, about, gel filtration of denatured and native enzyme Rattus norvegicus
68000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-

Reaction

Reaction Comment Organism Reaction ID
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid structure Rattus norvegicus
a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid active site on small subunit Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
renaturation as catalytically active enzyme after inactivation with 6-diazo-5-oxo-L-norleucine, requires glutathione or S-methyl derivative as a substrate ligand, circular dichroic spectra Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ donor: GSH S-substituted derivatives Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Rattus norvegicus peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ donor: GSH S-substituted derivatives Rattus norvegicus Wistar peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is 5-L-Glu-4-nitroanilide Rattus norvegicus Wistar peptide + 5-L-glutamyl amino acid
-
?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Rattus norvegicus Wistar peptide + 5-L-glutamyl amino acid
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
Rattus norvegicus 4-nitroaniline + 5-L-glutamylglycylglycine
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
Rattus norvegicus Wistar 4-nitroaniline + 5-L-glutamylglycylglycine
-
?

Subunits

Subunits Comment Organism
dimer 1 * 48000 + 1 * 20000, about, gel filtration of denatured and native enzyme Rattus norvegicus
More the active site resides on the small subunit Rattus norvegicus
More the enzyme is composed of two non-identical catalytically active subunits Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Rattus norvegicus