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Literature summary for 2.3.2.2 extracted from

  • Moriguchi, M.; Yamada, M.; Suenaga, S.; Tanaka, H.; Wakasugi, A.; Hatanaka, S.I.
    Partial purification and properties of gamma-glutamyltranspeptidase from mycelia of Morchella esculenta (1986), Arch. Microbiol., 144, 15-19.
    View publication on PubMed
Search for more literature for 2.3.2.2

Activating Compound

Activating Compound Comment Organism Structure
Maleate no stimulation Morchella esculenta
additional information phosphate, arsenate, carbonate Morchella esculenta

General Stability

General Stability Organism
dilution inactivates Morchella esculenta
glycerol, 5% v/v, stabilizes Morchella esculenta

Inhibitors

Inhibitors Comment Organism Structure
L- or D-Serine/borate
-
 Morchella esculenta
p-chloromercuribenzoate no inhibition Morchella esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information hydrolysis data Morchella esculenta

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not hydrolase Morchella esculenta
K+ not hydrolase Morchella esculenta
Mg2+ not hydrolase Morchella esculenta
additional information
-
 Morchella esculenta
Na+ not hydrolase Morchella esculenta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
 isoform III, gel filtration Morchella esculenta
155000
-
 isoform I, gel filtration Morchella esculenta
219000
-
 isoform II, gel filtration Morchella esculenta

Organism

Organism UniProt Comment Textmining
Morchella esculenta
-
 3 isoforms I, II and III
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Morchella esculenta

Purification (Commentary)

Purification (Comment) Organism
partial, 3 isoforms Morchella esculenta

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Morchella esculenta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Morchella esculenta

Storage Stability

Storage Stability Organism
-20°C, crude, at least 1 year Morchella esculenta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donors are Glu, alpha-L-Glu-L-Ala Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
(5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
5-L-glutamyl-4-nitroanilide + glycine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
5-L-glutamyl-4-nitroanilide + L-alanine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-aspartate
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
5-L-glutamyl-4-nitroanilide + L-cysteine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-cysteine
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
5-L-glutamyl-4-nitroanilide + L-isoleucine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
5-L-glutamyl-4-nitroanilide + L-lysine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
5-L-glutamyl-4-nitroanilide + L-methionine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
5-L-glutamyl-4-nitroanilide + L-proline
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
5-L-glutamyl-4-nitroanilide + L-serine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
5-L-glutamyl-4-nitroanilide + L-threonine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
5-L-glutamyl-4-nitroanilide + L-tryptophan
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
5-L-glutamyl-4-nitroanilide + L-valine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
5-L-glutamyl-cis-3-amino-L-proline + acceptor
-
 Morchella esculenta 5-L-glutamyl-acceptor + cis-3-amino-L-proline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 transpeptidation, isoform II and III Morchella esculenta
8.6
-
 transpeptidation, isoform I Morchella esculenta
8.6 9 hydrolase reaction, isoform III Morchella esculenta
9
-
 hydrolase reaction, isoform II Morchella esculenta
9 9.2 hydrolase reaction, isoform I Morchella esculenta