Application | Comment | Organism |
---|---|---|
nutrition | mutant enzyme D445A is suitable for fermentation of soy sauce and miso | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D445A | mutation abolishes transpeptidation activity, specific activity for hydrolysis is 40.2% of that of the wild-type enzyme, salt tolerant like the wild-type enzyme | Bacillus subtilis |
D445E | transpeptidation activity is 40% of hydrolysis activity, in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis |
D445N | transpeptidation activity is 42% of hydrolysis activity, in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis |
D445Y | transpeptidation activity is 40% of hydrolysis activity, in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis |
R113K | transpeptidation activity is 72% of hydrolysis activity, in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
5-L-glutamyl-4-nitroanilide | wild-type enzyme | Bacillus subtilis | |
15.3 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme D445A | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes from Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + glycylglycine | in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + H2O | in wild-type enzyme the transpeptidation activity is 2.38times higher than the hydrolysis activity | Bacillus subtilis | p-nitroaniline + Glu | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43.33 | - |
5-L-glutamyl-4-nitroanilide | wild-type enzyme | Bacillus subtilis | |
56.33 | - |
5-L-glutamyl-4-nitroanilide | mutant enzyme D445A | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | glutaminase activity of mutant enzyme D445A | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 40% of maximal activity, pH 10.0: about 35% of maximal activity, glutaminase activity of mutant enzyme D445A | Bacillus subtilis |