Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | specific for vicinal sulfhydryl sites in proteins, irreversible or very slow reversible, instead of other inhibition with arsenite which is usually rapidly reversed upon addition of competitive dithiol compounds | Oryctolagus cuniculus | |
casein | 71 microM inhibited by 50%, with 79 microM cytochrome c, competitive inhibition | Oryctolagus cuniculus | |
iodoacetamide | reacts slowly, relative insenstitive to iodoacetamide. This property distinguishes E2230K from other E2 proteins | Oryctolagus cuniculus | |
lysozyme | 139 microM inhibited by 90%, with 79 microM cytochrome c, competitive inhibition | Oryctolagus cuniculus | |
additional information | relatively insensitive to iodoacetamide inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
[histone H3]-L-lysine | pH 7.0, 37°C, Vmax, 0.06 microM | Oryctolagus cuniculus | |
0.125 | - |
[cytochrome c]-L-lysine | pH 7.0, 37°C, Vmax, 0.12 microM | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
gel fitration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification about 7fold with 20% recovery, gel filtration, ammonium sulfate fractionation, FPLC anion exchange on a Mono Q coloumn, E2230K is completely separated from other E2 proteins by the gel filtration step | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine | the enzyme forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and MgATP and catalyzes the conjugation of ubiquitin to protein substrates, independent of E3 | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
reticulocyte | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.81 | - |
pH 7.0, 37°C, purified enzyme, in the presence of 79 microM yeast cytochrome c and 0.1 microM E1 protein | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-lactoglobulin and reduced carboxymethylated bovine serum albumin rcmBSA are not conjugates | Oryctolagus cuniculus | ? | - |
? | |
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [alpha-casein]-L-lysine | 42% relative activity compared to cytochrome c as substrate | Oryctolagus cuniculus | [ubiquitin-carrier protein E2]-L-cysteine + [alpha-casein]-N6-ubiquitinyl-L-lysine | - |
? | |
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [alpha-lactalbumin]-L-lysine | 12% with human and 15% with bovine alpha-lactalbumin, relative activity compared to cytochrome c as substrate | Oryctolagus cuniculus | [ubiquitin-carrier protein E2]-L-cysteine + [alpha-lactalbumin]-N6-ubiquitinyl-L-lysine | - |
? | |
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [cytochrome c]-L-lysine | yeast cytochrome c, ubiquitin from human blood | Oryctolagus cuniculus | [ubiquitin-carrier protein E2]-L-cysteine + [cytochrome c]-N6-ubiquitinyl-L-lysine | - |
? | |
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [histone H3]-L-lysine | - |
Oryctolagus cuniculus | [ubiquitin-carrier protein E2]-L-cysteine + [histone H3]-N6-ubiquitinyl-L-lysine | - |
? | |
[ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [lysozyme]-L-lysine | 5% relative activity compared to cytochrome c as substrate | Oryctolagus cuniculus | [ubiquitin-carrier protein E2]-L-cysteine + [lysozyme]-N6-ubiquitinyl-L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 230000, SDS-PAGE | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
arsenite-sensitive E2 | - |
Oryctolagus cuniculus |
E2230K | the enzyme prepapration forms a labile addct with a molecular weight of ca. 230 kDa | Oryctolagus cuniculus |
UB carrier protein (E2) | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
[histone H3]-L-lysine | pH 7.0, 37°C, Vmax, 0.06 microM | Oryctolagus cuniculus | |
0.00617 | - |
[cytochrome c]-L-lysine | pH 7.0, 37°C, Vmax, 0.12 microM | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Oryctolagus cuniculus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and MgATP and catalyzes the conjugation of ubiquitin to protein substrates, independent of E3 | Oryctolagus cuniculus |