Literature summary for 2.3.2.27 extracted from

Yu, Y.; Xu, W.; Wang, J.; Wang, L.; Yao, W.; Yang, Y.; Xu, Y.; Ma, F.; Du, Y.; Wang, Y.
The Chinese wild grapevine (Vitis pseudoreticulata) E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1 (EIRP1) activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor (2013), New Phytol., 200, 834-846.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in onion epidermal cells and Arabidopsis thaliana	Vitis pseudoreticulata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell membrane	-	Vitis pseudoreticulata	-	-
cytoplasm	-	Vitis pseudoreticulata	5737	-
nucleus	-	Vitis pseudoreticulata	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26970	-	x * 26970, calculated from amino acid sequence	Vitis pseudoreticulata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine	Vitis pseudoreticulata	-	[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Vitis pseudoreticulata	-	Chinese wild grapevine, cultivar Carignane	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine	-	Vitis pseudoreticulata	[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine	-	?

Subunits

Subunits	Comment	Organism
?	x * 26970, calculated from amino acid sequence	Vitis pseudoreticulata

Synonyms

Synonyms	Comment	Organism
E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1	-	Vitis pseudoreticulata
EIRP1	-	Vitis pseudoreticulata

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vitis pseudoreticulata	calculated from amino acid sequence	-	5.6

Expression

Organism	Comment	Expression
Vitis pseudoreticulata	enzyme expression is induced by powdery mildew	up

General Information

General Information	Comment	Organism
physiological function	the enzyme activates plant defense responses by inducing proteolysis of the VpWRKY11 transcription factor. Enzyme overexpression in Arabidopsis confers enhanced resistance to the pathogens Golovinomyces cichoracearum and Pseudomonas syringae pv. tomato DC3000	Vitis pseudoreticulata

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Release 2023.1 (January 2023)