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Literature summary for 2.3.2.5 extracted from

  • Gololobov, M.Y.; Song, I.; Wang, W.; Bateman, R.C.M.
    Steady-state kinetics of glutamine cyclotransferase (1994), Arch. Biochem. Biophys., 309, 300-307.
    View publication on PubMed
Search for more literature for 2.3.2.5

Protein Variants

Protein Variants Comment Organism
additional information N53A and N123A: no expression of either of the mutants is detected Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.379
-
 L-glutamine tert-butyl ester pH 6.5 Carica papaya
0.4
-
 L-glutamine tert-butyl ester pH 6.75 Carica papaya
0.43
-
 L-glutamine tert-butyl ester pH 7.45 Carica papaya
0.44
-
 L-glutamine tert-butyl ester pH 8.05 Carica papaya
0.46
-
 L-glutamine tert-butyl ester pH 7.0 or pH 8.6 Carica papaya
0.55
-
 L-glutamine tert-butyl ester pH 6.2 Carica papaya
0.6
-
 L-glutamine tert-butyl ester pH 9.0 Carica papaya
0.62
-
 L-glutamine tert-butyl ester pH 6.3 Carica papaya
0.9
-
 L-glutamine tert-butyl ester pH 9.55 Carica papaya
1.3
-
 L-glutamine tert-butyl ester pH 9.95 Carica papaya

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Carica papaya
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32980
-
 x * 32980, native, glycosylated enzyme, SDS-PAGE Carica papaya
33000
-
 x * 33000, native, glycosylated enzyme, SDS-PAGE Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein presence of terminal mannose residues, mannose and N-acetylglucosamine in a 3:1 molar ratio Carica papaya

Purification (Commentary)

Purification (Comment) Organism
-
 Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine tert-butyl ester
-
 Carica papaya L-pyroglutamate tert-butyl ester + NH3
-
 ?
L-glutaminyl-peptide simple intramolecular cyclization. The mechanism consists of the following main steps: 1. intramolecular nucleophilic attack on the gamma-C=O carbon by the nitrogen of the alpha-amino group, 2. transfer of a proton from the alpha-amino group to the nitrogen of the amide group, facilitated by an acidic group of the enzyme, 3. expulsion of the ammonia-leaving group promoted by this or another acidic enzyme group Carica papaya 5-oxoprolyl-peptide + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 32980, native, glycosylated enzyme, SDS-PAGE Carica papaya
? x * 33000, native, glycosylated enzyme, SDS-PAGE Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 8.8, several hours, no inactivation Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 activity changes very slightly in the pH range between 4.5 and 10 Carica papaya