Literature summary for 2.3.2.5 extracted from

Cynis, H.; Rahfeld, J.U.; Stephan, A.; Kehlen, A.; Koch, B.; Wermann, M.; Demuth, H.U.; Schilling, S.
Isolation of an isoenzyme of human glutaminyl cyclase: retention in the Golgi complex suggests involvement in the protein maturation machinery (2008), J. Mol. Biol., 379, 966-980.

Search for more literature for 2.3.2.5

Cloned(Commentary)

Cloned (Comment)	Organism
h-isoQC, lacking the N-terminal signal anchor and the short cytosolic tail, is expressed as a fusion protein in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Homo sapiens
benzimidazole	-	Homo sapiens
benzylimidazole	-	Homo sapiens
cysteamine	-	Homo sapiens
EDTA	-	Homo sapiens
H-Gln-7-amido-4-methylcoumarin	substrate inhibition	Homo sapiens
H-Gln-beta-naphthylamide	substrate inhibition	Homo sapiens
imidazole	-	Homo sapiens
methylimidazole	-	Homo sapiens
N-dimethylcysteamine	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	H-Gln-7-amido-4-methylcoumarin	pH 8.0, 30°C	Homo sapiens
0.036	-	H-Gln-beta-naphthylamide	pH 8.0, 30°C	Homo sapiens
0.115	-	H-Gln-Gln-OH	pH 8.0, 30°C	Homo sapiens
0.214	-	H-Gln-Gly-Pro-OH	pH 8.0, 30°C	Homo sapiens
0.424	-	H-Gln-Gly-OH	pH 8.0, 30°C	Homo sapiens
0.705	-	H-Gln-Glu-OH	pH 8.0, 30°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	exclusively localized within the Golgi complex, obviously retained by the N-terminus. Retention in the Golgi complex suggests involvement in the protein maturation machinery	Homo sapiens	5794	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	ubiquitous expression	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gln-Gln	-	Homo sapiens	5-oxoprolyl-Gln + NH3	-	?
Gln-Gly	-	Homo sapiens	5-oxoprolyl-Gly + NH3	-	?
H-Gln-7-amido-4-methylcoumarin	-	Homo sapiens	5-oxoprolyl-7-amido-4-methylcoumarin + NH3	-	?
H-Gln-beta-naphthylamide	-	Homo sapiens	5-oxoprolyl-beta-naphthylamide + NH3	-	?
H-Gln-Glu-OH	-	Homo sapiens	5-oxoprolyl-Glu-OH + NH3	-	?
H-Gln-Gly-Pro-OH	-	Homo sapiens	5-oxoprolyl-Gly-Pro-OH + NH3	-	?

Synonyms

Synonyms	Comment	Organism
glutaminyl cyclase	-	Homo sapiens
h-isoQC	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.07	-	H-Gln-7-amido-4-methylcoumarin	pH 8.0, 30°C	Homo sapiens
1.66	-	H-Gln-Gly-OH	pH 8.0, 30°C	Homo sapiens
2.65	-	H-Gln-Glu-OH	pH 8.0, 30°C	Homo sapiens
2.72	-	H-Gln-Gln-OH	pH 8.0, 30°C	Homo sapiens
3.4	-	H-Gln-beta-naphthylamide	pH 8.0, 30°C	Homo sapiens
4.02	-	H-Gln-Gly-Pro-OH	pH 8.0, 30°C	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0073	-	benzylimidazole	pH 8.0, 30°C	Homo sapiens
0.022	-	N-dimethylcysteamine	pH 8.0, 30°C	Homo sapiens
0.068	-	cysteamine	pH 8.0, 30°C	Homo sapiens
0.079	-	methylimidazole	pH 8.0, 30°C	Homo sapiens
0.199	-	benzimidazole	pH 8.0, 30°C	Homo sapiens
0.219	-	imidazole	pH 8.0, 30°C	Homo sapiens
1.47	-	H-Gln-beta-naphthylamide	-	Homo sapiens
5.73	-	H-Gln-7-amido-4-methylcoumarin	-	Homo sapiens

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Release 2023.1 (January 2023)