Literature summary for 2.3.2.B1 extracted from

Lloyd, A.J.; Gilbey, A.M.; Blewett, A.M.; De Pascale, G.; El Zoeiby, A.; Levesque, R.C.; Catherwood, A.C.; Tomasz, A.; Bugg, T.D.H.; Roper, D.I.; Dowson, C.G.
Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan (2008), J. Biol. Chem., 283, 6402-6417.

Search for more literature for 2.3.2.B1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00021	-	L-alanyl-tRNA	pH 7.6, 37°C	Streptococcus pneumoniae
0.031	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine	pH 7.6, 37°C	Streptococcus pneumoniae
0.035	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.037	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.237	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	penicillin-resistant strain	-
Streptococcus pneumoniae	-	penicillin-susceptible strain	-
Streptococcus pneumoniae 159	-	penicillin-resistant strain	-
Streptococcus pneumoniae Pn16	-	penicillin-susceptible strain	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged recombinant protein	Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.172	-	substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C	Streptococcus pneumoniae
0.175	-	substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C	Streptococcus pneumoniae
0.247	-	substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C	Streptococcus pneumoniae
0.28	-	substrate L-seryl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C	Streptococcus pneumoniae
0.31	-	substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C	Streptococcus pneumoniae
0.37	-	substrate L-seryl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C	Streptococcus pneumoniae
1.92	-	substrate L-alanyl-tRNA from penicillin-resistant strain 159, pH 7.6, 37°C	Streptococcus pneumoniae
2.09	-	substrate L-alanyl-tRNA from penicillin-susceptible strain Pn16, pH 7.6, 37°C	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. lipid I	Streptococcus pneumoniae	? + tRNA	-	?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. lipid I	Streptococcus pneumoniae 159	? + tRNA	-	?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	i.e. lipid I	Streptococcus pneumoniae Pn16	? + tRNA	-	?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA	-	Streptococcus pneumoniae	? + tRNA	-	?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA	-	Streptococcus pneumoniae 159	? + tRNA	-	?
ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA	-	Streptococcus pneumoniae Pn16	? + tRNA	-	?
additional information	differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae	?	-	?
additional information	MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae	?	-	?
additional information	differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae 159	?	-	?
additional information	MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae 159	?	-	?
additional information	differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae Pn16	?	-	?
additional information	MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla	Streptococcus pneumoniae Pn16	?	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation	Streptococcus pneumoniae 159	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA	in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation	Streptococcus pneumoniae Pn16	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA	in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA	-	?
UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA	MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro	Streptococcus pneumoniae	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0031	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.0157	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine	pH 7.6, 37°C	Streptococcus pneumoniae
0.017	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.021	-	L-alanyl-tRNA	pH 7.6, 37°C	Streptococcus pneumoniae
0.033	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae

General Information

General Information	Comment	Organism
physiological function	adds L-serine or L-alanine as the first amino acid of a dipeptide branch to the stem peptide lysine of the pneumococcal peptidoglycan. MurM activity is essential for clinical pneumococcal penicillin resistance	Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.013	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.1	-	L-alanyl-tRNA	pH 7.6, 37°C	Streptococcus pneumoniae
0.205	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae
0.46	-	ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine	pH 7.6, 37°C	Streptococcus pneumoniae
0.948	-	UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine	pH 7.6, 37°C	Streptococcus pneumoniae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)