Literature summary for 2.3.2.B8 extracted from

Kamadurai, H.B.; Souphron, J.; Scott, D.C.; Duda, D.M.; Miller, D.J.; Stringer, D.; Piper, R.C.; Schulman, B.A.
Insights into ubiquitin transfer cascades from a structure of a UbcH5B approximately ubiquitin-HECT(NEDD4L) complex (2009), Mol. Cell., 36, 1095-1102.

Crystallization (Commentary)

Crystallization (Comment)	Organism
determination of the crystal of a complex between the HECT domain NEDD4L and the E2 UbcH5B bearing a covantly-linked ubiquitin at its active site to gain insights of the ubiquitin transfer from an associated E2 to the acceptor cysteine in the HECT domain C-lobe. HECT contains is bilobed, consists of N-terminal N-lobe and C-terminal C-lobe. The C-lobe is essential for the E2-to-E3 ubiquitin transfer and the N-lobe is essential for the the further protein substrate processivity, the transfer of the ubiquitin from E3 to the substrate	Homo sapiens

Crystallization (Comment)

Organism

determination of the crystal of a complex between the HECT domain NEDD4L and the E2 UbcH5B bearing a covantly-linked ubiquitin at its active site to gain insights of the ubiquitin transfer from an associated E2 to the acceptor cysteine in the HECT domain C-lobe. HECT contains is bilobed, consists of N-terminal N-lobe and C-terminal C-lobe. The C-lobe is essential for the E2-to-E3 ubiquitin transfer and the N-lobe is essential for the the further protein substrate processivity, the transfer of the ubiquitin from E3 to the substrate

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P62837	-	-
Homo sapiens	Q96PU5	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine = [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine	the 40 kDa C-terminal HET domain binds a reactive thioester-linked E2-ubiquitin covalent-linked complex. The ubiqiutin is transferred from the E2 catalytic cysteine to the HECT domain catalytic cysteine via a transthiolation reaction	Homo sapiens	a

Synonyms

Synonyms	Comment	Organism
E3 HECT	-	Homo sapiens
E3 ubiquitin-protein ligase	-	Homo sapiens
UbcH5B-ubiquitin-HECT NEDD4L complex	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	part of the E1-E2-E3 ubiquitin conjugation cascade. The E2-ubiquitin covalent complex interacts with an E3 (HECT) for ubiquitin transfer. Ubiquitin is transferred to the acceptor cysteine of the C-lobe of the HECT domain	Homo sapiens