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Literature summary for 2.3.2.B8 extracted from

  • Riling, C.; Kamadurai, H.; Kumar, S.; OLeary, C.E.; Wu, K.P.; Manion, E.E.; Ying, M.; Schulman, B.A.; Oliver, P.M.
    Itch WW domains inhibit its E3 ubiquitin ligase activity by blocking E2-E3 ligase trans-thiolation (2015), J. Biol. Chem., 290, 23875-23887.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.B8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and HEK-293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
R600A this HECT-domain mutant is inactive Mus musculus
Y763A this HECT-domain mutant is inactive Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 x * 100000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine Mus musculus
-
 [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8C863
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography or glutathione Sepharose column chromatography and gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is autoinhibited by an intramolecular interaction between its HECT (homologous to the E6-AP carboxy terminus domain) and two central WW domains. Ndfip1 binds these WW domains to release the HECT, allowing trans-thiolation and enzyme catalytic activity Mus musculus ?
-
 ?
S-ubiquitinyl-[ubiquitin-carrier protein E2]-L-cysteine + [HECT-E3-ubiquitin-carrier protein]-L-cysteine
-
 Mus musculus [ubiquitin-carrier protein E2]-L-cysteine + S-ubiquitinyl-[HECT-E3-ubiquitin-carrier protein]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
E2-E3 ligase
-
 Mus musculus
Itch
-
 Mus musculus