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Literature summary for 2.3.3.10 extracted from

  • Clinkenbeard, K.D.; Sugiyama, T.; Lane, M.D.
    Cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase from chicken liver (1975), Methods Enzymol., 35 B, 160-167.
No PubMed abstract available
Search for more literature for 2.3.3.10

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) Ellman's reagent Gallus gallus
5,5'-dithiobis(2-nitrobenzoate)
-
 Saccharomyces cerevisiae
acetoacetyl-CoA
-
 Gallus gallus
acetoacetyl-CoA
-
 Saccharomyces cerevisiae
p-chloromercuribenzoate
-
 Gallus gallus
p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 acetyl-CoA 0.1 mM acetoacetyl-CoA Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Gallus gallus 5737
-
cytoplasm
-
 Saccharomyces cerevisiae 5737
-
cytosol
-
 Gallus gallus 5829
-
cytosol
-
 Saccharomyces cerevisiae 5829
-
mitochondrion
-
 Gallus gallus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, synthase II, SDS-PAGE Gallus gallus
100000
-
 cytosolic enzyme, sedimentation equilibrium Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus cytosolic synthase catalyzes the second step of cholesterogenesis from acetyl-CoA (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 chicken
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic synthase Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3'-dephospho-CoA + H2O
-
 Gallus gallus acetyldephospho-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O cytosolic synthase catalyzes the second step of cholesterogenesis from acetyl-CoA Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O mitochondrial enzyme of liver functions in ketogenesis, its cytoplasmic counterpart participates in cholesterogenesis Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 55000, synthase II, SDS-PAGE Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
8 9.6
-
 Gallus gallus