General Stability | Organism |
---|---|
enzyme is labile to freezing and thawing, stabilized by glycerol | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Saccharomyces cerevisiae | |
Cd2+ | - |
Saccharomyces cerevisiae | |
CoA | - |
Saccharomyces cerevisiae | |
iodoacetamide | - |
Saccharomyces cerevisiae | |
iodoacetate | - |
Saccharomyces cerevisiae | |
N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae | |
0.014 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
0.018 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Saccharomyces cerevisiae | - |
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°, stored in presence of dithiothreitol and 30-50% glycerol purified enzyme retains complete activity for up to 3 months | Saccharomyces cerevisiae |
4°C, enzyme loses activity in absence of glycerol | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA | - |
? | |
acetyl-CoA + acetoacetyl-ACP + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-ACP | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetylglutathione + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
acetylpantetheine + acetoacetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-3-methylglutaryl-CoA + pantetheine | - |
? | |
additional information | absolutely specific for the acyl-moiety of both its substrates, neither propionyl- nor butyryl CoA can replace acetyl-CoA | Saccharomyces cerevisiae | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
acetoacetyl-CoA | pH 8.9 | Saccharomyces cerevisiae |