General Stability | Organism |
---|---|
relatively stable dialyzed against 10 mM potassium phosphate in 0.1 mM dithiothreitol and EDTA for 4-10 days | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetyl-CoA | 0.005 mM acetoacetyl-CoA | Gallus gallus | |
0.5 | - |
acetyl-CoA | 0.05 mM acetoacetyl-CoA | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Gallus gallus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Gallus gallus |
105000 | - |
sedimentation equlibrium | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | Gallus gallus | potentially favorable position for the regulation of hepatic ketogenesis | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, may be stored in 30% glycerin solution for 2-3 months with no loss of activity | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
acetyl-CoA + acetoacetyl-CoA + H2O | potentially favorable position for the regulation of hepatic ketogenesis | Gallus gallus | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Gallus gallus |