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Literature summary for 2.3.3.10 extracted from

  • Barrero, M.J.; Alho, C.S.; Ortiz, J.A.; Hegardt, F.G.; Haro, D.; Marrero, P.F.
    Low activity of mitochondrial HMG-CoA synthase in liver of starved pigletsis due to low levels of protein despite high mRNA levels (2001), Arch. Biochem. Biophys., 385, 364-371.
    View publication on PubMed
Search for more literature for 2.3.3.10

Cloned(Commentary)

Cloned (Comment) Organism
pig mitochondrial HMG-CoA synthase encodes an active enzyme in the eukaryotic cell line Mev-1 and in Escherichia coli BL21 (DE3) Sus scrofa
rat mitochondrial HMG-CoA synthase encodes an active enzyme in the eukaryotic cell line Mev-1 in Escherichia coli BL21 (DE3) Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.695
-
 acetyl-CoA
-
 Sus scrofa
0.811
-
 acetyl-CoA
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 recombinant enzyme, epressed in Mev-1 cells Sus scrofa
56000
-
 recombinant enzyme, epressed in Escherichia coli Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O Sus scrofa pathway converting acetyl-CoA to ketone bodies (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus Sprague-Dawley
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-
Sus scrofa
-
 piglet
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
acetyl-CoA + acetoacetyl-CoA + H2O pathway converting acetyl-CoA to ketone bodies Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus Sprague-Dawley (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?