Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with (S)-3-hydroxy-3-methylglutaryl-CoA, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 6.5), 25% (w/v) PEG 3350, at 20°C | Homo sapiens |
in complex with Co-A, sitting drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 25% (w/v) polyethylene glycol (PEG) 3350, 0.2 M ammonium sulfate, at 4°C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F174L | the mutant shows 10000fold decrease of activity compared to the wild type enzyme | Homo sapiens |
G169D | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
G212R | inactive | Homo sapiens |
G388R | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
I407T | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
I56N | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
K243E | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
L266S | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
M307T | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
R188H | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
R424X | the truncated mutant is nonfunctional | Homo sapiens |
R500H | inactive | Homo sapiens |
R505Q | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
T233A | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
V54M | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
Y167C | the mutant is associated with HMGCS2 deficiency | Homo sapiens |
Y84X | the truncated mutant is nonfunctional | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform HMGCS1 | Homo sapiens | 5829 | - |
mitochondrion | isoform HMGCS2 | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P54868 | isoform HMGCS2 | - |
Homo sapiens | Q01581 | isoform HMGCS1 | - |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose resin column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetoacetyl-CoA + H2O | - |
Homo sapiens | (S)-3-hydroxy-3-methylglutaryl-CoA + CoA | - |
? | |
additional information | the enzyme catalyzes a three-step ping-pong reaction involving an acylation, a condensation, and a hydrolysis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl coenzyme A synthase | - |
Homo sapiens |
HMG-CoA synthase | - |
Homo sapiens |
HMGCS | - |
Homo sapiens |
HMGCS1 | isoform | Homo sapiens |
HMGCS2 | isoform | Homo sapiens |