Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.10 extracted from

  • Wang, H.; Nagegowda, D.A.; Rawat, R.; Bouvier-Nave, P.; Guo, D.; Bach, T.J.; Chye, M.L.
    Overexpression of Brassica juncea wild-type and mutant HMG-CoA synthase 1 in Arabidopsis up-regulates genes in sterol biosynthesis and enhances sterol production and stress tolerance (2012), Plant Biotechnol. J., 10, 31-42.
    View publication on PubMed
Search for more literature for 2.3.3.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Arabidopsis thaliana Brassica juncea

Protein Variants

Protein Variants Comment Organism
H188N the mutant lacks substrate inhibition by acetoacetyl-CoA and shows 8fold decreased enzyme activity Brassica juncea
H188N/S359A the double mutant displays a 10fold increased enzyme activity and lacks inhibition by acetoacetyl-CoA Brassica juncea
S359A the mutant demonstrates 10fold higher activity as the wild type enzyme Brassica juncea

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA substrate inhibition Brassica juncea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Brassica juncea 5829
-

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + acetoacetyl-CoA + H2O
-
 Brassica juncea (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl-CoA synthase
-
 Brassica juncea
HMG-CoA synthase 1
-
 Brassica juncea
HMGS
-
 Brassica juncea

General Information

General Information Comment Organism
metabolism 3-hydroxy-3-methylglutaryl-CoA synthase is the second enzyme in the cytosolic mevalonate pathway Brassica juncea
physiological function enzyme overexpression up-regulates 3-hydroxy-3-methylglutaryl-CoA reductase, sterol methyltransferase 2, delta-24 sterol reductase, C-22 sterol desaturase, and brassinosteroid-6-oxidase 2, leading to enhanced sterol content and stress tolerance in Arabidopsis. Enzyme overexpressing Arabidopsis seeds germinate approximately 1 day earlier than wild type seeds. Enzyme overexpressing Arabidopsis seedlings show enhanced resistance to Botrytis cinerea, and detached leaves are delayed in H2O2-induced cell death Brassica juncea