Cloned (Comment) | Organism |
---|---|
H37Rv leuA gene with and without the tandem repeats is cloned by PCR and expressed in an Escherichia coli host. The 57-bp tandem repeats located in the leuA gene code for the alpha-isopropylmalate synthase. Deletion of the repeats has no detectable effect on leuA expression level or the general properties of the enzyme product | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 50% of maximal activity, 60°C: about 40% of maximal activity | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | stability of the mutated alpha-IPMS is similar to that of the native alpha-IPMS in that keeping the enzyme at pH 7.5 is better than at pH 4.0 | Mycobacterium tuberculosis |