Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Rosetta cells | Solanum lycopersicum |
expressed in Escherichia coli BL21 Rosetta cells | Solanum pennellii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | - |
Solanum lycopersicum | |
L-leucine | - |
Solanum pennellii | |
additional information | isoform IPMS3 is not subject to L-leucine feedback inhibition; isoform IPMS3 is not subject to L-leucine feedback inhibition | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.143 | - |
2-oxoisovalerate | isoform IPMS1, pH and temperature not specified in the publication | Solanum lycopersicum | |
0.215 | - |
2-oxoisovalerate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum | |
0.734 | - |
4-methylthio-2-oxobutyrate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Solanum lycopersicum | - |
(2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Solanum pennellii | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | K4C627 | isoform IPMS1; M82 | - |
Solanum lycopersicum | K4C627 | isoform IPMS2; M82 | - |
Solanum lycopersicum | K4CJ56 | isoform IPMS3; M82 | - |
Solanum pennellii | A0A0G2T6D7 | isoform IPMS3; LA071 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Solanum lycopersicum |
Ni-NTA column chromatography | Solanum pennellii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Solanum lycopersicum | - |
stem | - |
Solanum pennellii | - |
trichome | - |
Solanum lycopersicum | - |
trichome | - |
Solanum pennellii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-oxoisovalerate + H2O | - |
Solanum lycopersicum | ? | - |
? | |
acetyl-CoA + 2-oxoisovalerate + H2O | - |
Solanum pennellii | ? | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Solanum lycopersicum | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Solanum pennellii | (2S)-2-isopropylmalate + CoA | - |
? | |
acetyl-CoA + 4-methylthio-2-oxobutyrate + H2O | - |
Solanum lycopersicum | ? | - |
? | |
acetyl-CoA + 4-methylthio-2-oxobutyrate + H2O | - |
Solanum pennellii | ? | - |
? | |
additional information | isoform IPMS3 shows no activity with 4-methylthio-2-oxobutyrate or 2-oxoisovalerate | Solanum pennellii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMS1 | isoform | Solanum lycopersicum |
IPMS2 | isoform | Solanum lycopersicum |
IPMS3 | isoform | Solanum pennellii |
IPMS3 | isoform | Solanum lycopersicum |
isopropylmalate synthase | - |
Solanum lycopersicum |
isopropylmalate synthase | - |
Solanum pennellii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
4-methylthio-2-oxobutyrate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum | |
0.49 | - |
2-oxoisovalerate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum | |
1.08 | - |
2-oxoisovalerate | isoform IPMS1, pH and temperature not specified in the publication | Solanum lycopersicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
4-methylthio-2-oxobutyrate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum | |
2.3 | - |
2-oxoisovalerate | isoform IPMS3, pH and temperature not specified in the publication | Solanum lycopersicum | |
7.5 | - |
2-oxoisovalerate | isoform IPMS1, pH and temperature not specified in the publication | Solanum lycopersicum |