Cloned (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
the structure of SpHCS in complex with the inhibitor L-lysine and Zn2+ is solved at a resolution of 2.38 A | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
D123N | mutant | Schizosaccharomyces pombe |
E222Q | mutant | Schizosaccharomyces pombe |
Q364R | mutant | Schizosaccharomyces pombe |
R288K | mutant | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | feedback inhibition | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0107 | - |
acetyl-CoA | wild-type | Schizosaccharomyces pombe | |
0.0132 | - |
acetyl-CoA | mutant D123N | Schizosaccharomyces pombe | |
0.014 | - |
acetyl-CoA | mutant R288K | Schizosaccharomyces pombe | |
0.0153 | - |
acetyl-CoA | mutant Q364R | Schizosaccharomyces pombe | |
0.0477 | - |
acetyl-CoA | mutant E222Q | Schizosaccharomyces pombe | |
0.159 | - |
2-oxoglutarate | wild-type | Schizosaccharomyces pombe | |
0.247 | - |
2-oxoglutarate | mutant R288K | Schizosaccharomyces pombe | |
0.284 | - |
2-oxoglutarate | mutant D123N | Schizosaccharomyces pombe | |
0.296 | - |
2-oxoglutarate | mutant Q364R | Schizosaccharomyces pombe | |
9.66 | - |
2-oxoglutarate | mutant E222Q | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | Schizosaccharomyces pombe | - |
(R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9Y823 | - |
- |
Purification (Comment) | Organism |
---|---|
on Talon or Zn2+ charged IMAC sepharose resin | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | - |
Schizosaccharomyces pombe | (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HCS | - |
Schizosaccharomyces pombe |
homocitrate synthase | - |
Schizosaccharomyces pombe |
SpHCS | - |
Schizosaccharomyces pombe |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.75 | - |
2-oxoglutarate | mutant Q364R | Schizosaccharomyces pombe | |
2.833 | - |
acetyl-CoA | mutant Q364R | Schizosaccharomyces pombe | |
3.183 | - |
2-oxoglutarate | mutant E222Q | Schizosaccharomyces pombe | |
3.233 | - |
acetyl-CoA | mutant R288K | Schizosaccharomyces pombe | |
3.333 | - |
2-oxoglutarate | mutant R288K | Schizosaccharomyces pombe | |
3.583 | - |
acetyl-CoA | mutant E222Q | Schizosaccharomyces pombe | |
3.617 | - |
acetyl-CoA | mutant D123N | Schizosaccharomyces pombe | |
3.617 | - |
2-oxoglutarate | mutant D123N | Schizosaccharomyces pombe | |
4.983 | - |
acetyl-CoA | wild-type | Schizosaccharomyces pombe | |
5.133 | - |
2-oxoglutarate | wild-type | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Schizosaccharomyces pombe |