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Literature summary for 2.3.3.14 extracted from

  • Kumar, V.P.; West, A.H.; Cook, P.F.
    Kinetic and chemical mechanisms of homocitrate synthase from Thermus thermophilus (2011), J. Biol. Chem., 286, 29428-29439.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) RIL star cells Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
CoA in the presence of Mg2+, or Mn2+, product inhibition by CoA is competitive versus acetyl-CoA but uncompetitive versus 2-oxoglutarate Thermus thermophilus
EDTA
-
 Thermus thermophilus
L-lysine competitive inhibitor versus 2-oxoglutarate up to a concentration of 1 mM Thermus thermophilus
additional information not inhibited by oxalate, malonyl-CoA, pantothenate Thermus thermophilus
oxalglycine competitive inhibition versus 2-oxoglutarate Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Thermus thermophilus
Mn2+ activates Thermus thermophilus
additional information not activated by 50 mM Zn2+, 5 mM Ni2+ and 5 mM Co2+ Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate
-
 Thermus thermophilus 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
acetyl-CoA + H2O + oxaloacetate oxaloacetate is a slow alternative substrate Thermus thermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
HCS
-
 Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermus thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
 CoA competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus
0.11
-
 CoA competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus
0.9
-
 oxalglycine in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus