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Literature summary for 2.3.3.16 extracted from
- Purification and some properties of citrate synthase from Penicillium spiculisporum (1972), Eur. J. Biochem., 29, 60-66.
General Stability
| General Stability | Organism |
|---|---|
| very labile, 97% loss of activity after 2 d at 0°C due to aggregation | Talaromyces trachyspermus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | competitive against acetyl-CoA, inhibition reduced by Mg2+ | Talaromyces trachyspermus |  |
| Dithionitrobenzoate | - |
Talaromyces trachyspermus | |
| iodoacetamide | - |
Talaromyces trachyspermus | |
| lauroyl-CoA | - |
Talaromyces trachyspermus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
oxaloacetate | in presence of KCl | Talaromyces trachyspermus | |
| 0.03 | - |
acetyl-CoA | - |
Talaromyces trachyspermus | |
| 0.05 | - |
acetyl-CoA | in presence of 2.5 mM ATP | Talaromyces trachyspermus | |
| 0.07 | - |
oxaloacetate | - |
Talaromyces trachyspermus | |
| 0.08 | - |
acetyl-CoA | in presence of 5 mM ATP | Talaromyces trachyspermus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | reduces ATP inhibition | Talaromyces trachyspermus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | 70000 | gel filtration | Talaromyces trachyspermus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces trachyspermus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Talaromyces trachyspermus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | partially purified enzyme | Talaromyces trachyspermus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Talaromyces trachyspermus | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay at | Talaromyces trachyspermus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
enzyme assay at | Talaromyces trachyspermus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
ATP | - |
Talaromyces trachyspermus | |
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Release 2023.1 (January 2023)
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