Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli JM105, DNA and amino acid sequence analysis | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
acetyl-CoA | recombinant enzyme | Pyrococcus furiosus | |
0.005 | - |
oxaloacetate | recombinant enzyme | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 5fold increase at 100-200 mM | Pyrococcus furiosus | |
NaCl | 5fold increase at 100-200 mM | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Pyrococcus furiosus |
additional information | - |
secondary and tertiary structure | Pyrococcus furiosus |
42600 | - |
2 * 42600, SDS-PAGE | Pyrococcus furiosus |
89700 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from E. coli | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme from E. coli | Pyrococcus furiosus |
112.5 | - |
recombinant enzyme | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Pyrococcus furiosus | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42600, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
enzyme assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 20 min | Pyrococcus furiosus |
104 | - |
half-life: 1 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme assay at | Pyrococcus furiosus |