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Literature summary for 2.3.3.16 extracted from

  • Belova, L.L.; Sokolov, A.P.; Morgunov, I.G.; Trotsenko, YuA.
    Purification and characterization of citrate synthase from Methylobacterium extorquens - a methylotrophic producer of polyhydroxybutyrate (1997), Biochemistry, 62, 71-76.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate no inhibition Methylorubrum extorquens
ADP no inhibition Methylorubrum extorquens
ATP
-
Methylorubrum extorquens
NADH no effect Methylorubrum extorquens
NADPH no inhibition Methylorubrum extorquens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
oxaloacetate
-
Methylorubrum extorquens
0.084
-
acetyl-CoA
-
Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
KCl up to 80 mM Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
4 * 66000, SDS-PAGE Methylorubrum extorquens
260000
-
gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O Methylorubrum extorquens involved in poly 3-hydroxybutyrate synthesis citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylorubrum extorquens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
purified enzyme Methylorubrum extorquens

Storage Stability

Storage Stability Organism
4°C, Tris-HCl buffer, pH8.0, 50 mM glycerol, at least 1 week Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Methylorubrum extorquens citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O involved in poly 3-hydroxybutyrate synthesis Methylorubrum extorquens citrate + CoA
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 66000, SDS-PAGE Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
CS
-
Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme assay at Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6 9
-
Methylorubrum extorquens