Literature summary for 2.3.3.16 extracted from

Kocabiyik, S.; Erduran, I.
The effect of valine substitution for glycine in the dimer interface of citrate synthase from Thermoplasma acidophilum on stability and activity (2000), Biochem. Biophys. Res. Commun., 275, 460-465.

Search for more literature for 2.3.3.16

Activating Compound

Activating Compound	Comment	Organism	Structure
Guanidinium chloride	activation of recombinant wild-type 1.6fold at 0.5 M, inactivation at 1.6 M	Thermoplasma acidophilum
Urea	activation of recombinant wild-type by 35% at up to 8 M, inactivation at 9.3 M	Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant in Escherichia coli	Thermoplasma acidophilum

Protein Variants

Protein Variants	Comment	Organism
G196V	site-directed mutagenesis, mutation interferes with dimerization, improper dimerization or dissociation of the dimer, reduced enzyme activity and conformational stability	Thermoplasma acidophilum

Inhibitors

Inhibitors	Comment	Organism	Structure
Guanidinium chloride	irreversible inactivation of recombinant wild-type at 1.6 M and of recombinant mutant G196V at 0.2 M, at 0.5 M activation of the wild-type	Thermoplasma acidophilum
Urea	irreversible inactivation of recombinant wild-type at 9.3 M and of recombinant mutant G196V at 5 M, at up to 8 M activation of the wild-type	Thermoplasma acidophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0045	-	acetyl-CoA	recombinant mutant G196V	Thermoplasma acidophilum
0.005	-	acetyl-CoA	recombinant wild-type	Thermoplasma acidophilum
0.0057	-	oxaloacetate	recombinant mutant G196V	Thermoplasma acidophilum
0.0066	-	oxaloacetate	recombinant wild-type	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant G196V from E. coli	Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.008	-	recombinant purified mutant G196V	Thermoplasma acidophilum
0.083	-	recombinant purified wild-type	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + oxaloacetate + H2O	-	Thermoplasma acidophilum	citrate + CoA	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Thermoplasma acidophilum

Synonyms

Synonyms	Comment	Organism
CS	-	Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	enzyme assay at	Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	t1/2 of recombinant wild-type is 10 min, t1/2 of recombinant mutant G196V is 8 min, inactivation of the mutant occurs in 2 stages: a first slow and a second rapid one	Thermoplasma acidophilum
90	-	mutant G196V: t1/2: below 1 min, t1/2 wild-type: 5 min	Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0133	-	acetyl-CoA	recombinant mutant G196V	Thermoplasma acidophilum
0.15	-	acetyl-CoA	recombinant wild-type	Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	enzyme assay at	Thermoplasma acidophilum

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Release 2023.1 (January 2023)