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Literature summary for 2.3.3.16 extracted from

  • Koyama, H.; Kawamura, A.; Kihara, T.; Hara, T.; Takita, E.; Shibata, D.
    Overexpression of mitochondrial citrate synthase in Arabidopsis thaliana improved growth on a phosphorus-limited soil (2000), Plant Cell Physiol., 41, 1030-1037.
    View publication on PubMed
Search for more literature for 2.3.3.16

Application

Application Comment Organism
agriculture
-
 Daucus carota
agriculture overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots Arabidopsis thaliana
agriculture overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots Daucus carota

Cloned(Commentary)

Cloned (Comment) Organism
ectopic overexpression in Arabidopsis thaliana strain WS via infection with Agrobacterium tumefaciens, DNA sequence determination Daucus carota

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Arabidopsis thaliana plants ectopically overexpressing the Daucus carota citrate synthase gene via transformation with Agrobacterium tumefaciens, transgenic plants process the enzyme to its mature form, targeting into mitochondria, increased growth and phosphate accumulation Daucus carota
additional information construction of transgenic Arabidopsis thaliana plants via Agrobacterium infection, functional expression and targeting to the mitochondria Daucus carota

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Daucus carota 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O Arabidopsis thaliana
-
 citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O Daucus carota
-
 citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O Arabidopsis thaliana WS
-
 citrate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana WS
-
-
-
Daucus carota
-
-
-
Daucus carota O80433
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root wild-type and transgenis plants, the latter expressing the Daucus carota enzyme Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00023 0.00028 wild-type Arabidopsis thaliana
0.00078
-
 recombinant in transgenic Arabidopsis thaliana plants Daucus carota
0.23 0.28
-
 Arabidopsis thaliana
0.78
-
 transgenic Arabidopsis thaliana plants overexpressing the Daucus carota citrate synthase Daucus carota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Arabidopsis thaliana citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Daucus carota citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Arabidopsis thaliana WS citrate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
CS
-
 Arabidopsis thaliana
CS
-
 Daucus carota