KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
oxaloacetate | dimeric enzyme form with propionyl-CoA | Rhodothermus marinus | |
0.003 | - |
propionyl-CoA | dimeric enzyme form | Rhodothermus marinus | |
0.003 | - |
oxaloacetate | dimeric enzyme form with acetyl-CoA | Rhodothermus marinus | |
0.006 | - |
oxaloacetate | hexameric enzyme form | Rhodothermus marinus | |
0.007 | - |
acetyl-CoA | dimeric enzyme form | Rhodothermus marinus | |
0.012 | - |
acetyl-CoA | dimeric enzyme form | Rhodothermus marinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
N-terminal protein sequence | Rhodothermus marinus |
42000 | - |
2 * 42000, dimeric enzyme form, SDS-PAGE | Rhodothermus marinus |
45000 | - |
6 * 45000, hexameric enzyme form | Rhodothermus marinus |
100000 | - |
gel filtration | Rhodothermus marinus |
100000 | - |
dimeric enzyme form | Rhodothermus marinus |
280000 | - |
gel filtration | Rhodothermus marinus |
280000 | - |
hexameric enzyme form | Rhodothermus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | - |
2 enzyme forms | - |
Rhodothermus marinus | - |
strain DSM 4252 | - |
Purification (Comment) | Organism |
---|---|
2 enzyme forms | Rhodothermus marinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
purified dimeric and hexameric enzyme form | Rhodothermus marinus |
60 | - |
dimeric and hexameric enzyme | Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Rhodothermus marinus | citrate + CoA | - |
? | |
propionyl-CoA + oxaloacetate + H2O | only the dimeric enzyme form, no activity with the hexameric enzyme form | Rhodothermus marinus | 2-methylcitrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, dimeric enzyme form, SDS-PAGE | Rhodothermus marinus |
hexamer | 6 * 45000, hexameric enzyme form | Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
enzyme assay at | Rhodothermus marinus |
85 | - |
dimeric and hexameric enzyme forms | Rhodothermus marinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | - |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
hexameric enzyme form, half-life: 18 min, dimeric enzyme form, half-life: 12 min | Rhodothermus marinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
propionyl-CoA | dimeric enzyme form | Rhodothermus marinus | |
21 | - |
acetyl-CoA | hexameric enzyme form | Rhodothermus marinus | |
22 | - |
acetyl-CoA | dimeric enzyme form | Rhodothermus marinus |