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Literature summary for 2.3.3.16 extracted from
- Probing the Sinorhizobium meliloti-alfalfa symbiosis using temperature-sensitive and impaired-function citrate synthase mutants (2005), Mol. Plant Microbe Interact., 18, 134-141.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | construction of mutants with reduced enzymic activity by localized random PCR mutagenesis. In symbiosis with alfalfa, alfalfa plants form fully effective nodules when infected with Sinorhizobium meliloti mutants having as low as 7% of citrate synthase activity. Mutants with about 3% of wild-type citrate synthase activity form nodules with lower nitrogenase activity and a mutant with 0.5% of wild-type activity forms Fix-nodules | Sinorhizobium meliloti |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of mutants with reduced enzymic activity by localized random PCR mutagenesis. In symbiosis with alfalfa, alfalfa plants form fully effective nodules when infected with Sinorhizobium meliloti mutants having as low as 7% of citrate synthase activity. Mutants with about 3% of wild-type citrate synthase activity form nodules with lower nitrogenase activity and a mutant with 0.5% of wild-type activity forms Fix-nodules | Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sinorhizobium meliloti | citrate synthase is essential for nodule maintenance in infection of alfalfa | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | citrate synthase is essential for nodule maintenance in infection of alfalfa | Sinorhizobium meliloti | ? | - |
? | |
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Release 2023.1 (January 2023)
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